Scientifica HoloStim-3D

Fig. 1. (a) HoloStim-3D targeted cells within a mouse brain, with cells transfected via viral vector, and expressing GCaMP-6S and C1V1 for photostimulation. In the total FOV (745 μm), 100 cells were selected within an area of 450 μm and targeted for photostimulation with a spiral spot of approximately 6 mW/cell. (b) Each cell is identified with the target label and the corresponding activity observing during imaging with the resonant scanning mirror is shown in the line plots in Fig. 1c,d. Simultaneous photostimulation of each cell was activated 10 times following a 10 s lead-in over a total time of 110 seconds. The photostimulation was activated for 250 μs and the power was controlled via software control connected to a total power control (TPC) module onboard the Amplitude Satsuma (20 W output). Data acquired by Kelly Sakaki (Scientifica), Robert Lees and Adam Packer (both University of Oxford).

Fig. 2. Using most of the same conditions as the results seen in Fig. 1(a-d), the SLM targeted cells within a mouse brain, with cells transfected via viral vector, and expressing GCaMP-6S and C1V1 for photostimulation. In this example, 21 cells were selected within an area of 250 μm and targeted for photostimulation with a spiral spot of approximately 6 mW/cell. Data captured in-vivo using Scientifica's HyperScope RGG-GG multiphoton imaging system with HoloStim-3D and a 16x objective. Data acquired by Kelly Sakaki (Scientifica), Robert Lees and Adam Packer (both University of Oxford).

Fig. 3: All-optical interrogation of neural circuits: neurons are stimulated by multiple beamlets that draw spirals over the soma. Activated neurons generate action potentials.