Scientifica HyperScope | Two-Photon Microscopy and Photostimulation

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Scientifica HyperScope

Simultaneously perform two-photon microscopy and photostimulation with exceptional performance thanks to our most advanced multiphoton imaging system yet.

Available with a variety of scanning configurations, the HyperScope helps to answer complex research questions in either in vitro or in vivo samples.

The HyperScope is capable of three-photon imaging. See some of the data acquired using the HyperScope and MDU XL here.

Dual scan head

Two light paths enable simultaneous two-photon imaging and uncaging with exceptional performance.

Various scanning arrangements

The scan mirrors on the imaging path can be arranged as a galvo-galvo, resonant-galvo or galvo-galvo-resonant scan head.

Extended wavelength range

The HyperScope optics are coated for wavelengths up to 1400 nm to give you more experimental flexibility.

Image up to 4 dyes

Compatible with ChromoFlex to enable two-photon imaging of up to four dyes with highly-sensitive GaAsP photomultiplier tubes.

Deep imaging

Outstanding images of the finest structures deep within your sample. Combine with optical sectioning to produce excellent three-dimensional models.

Pioneering slim design

The compact footprint and flexible design of the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easy access to the light path

Simply remove the cover of the scan head to gain access to the light path.

Optional side port

If one of the scanning paths is not mounted, then you can gain access to an optional side port for increased functionality.

HyperScope in vitro configuration 360° view

These measurements were taken with two HyperScope systems, employing a Nikon 16X 0.8 NA 3 mm WD objective at 850 nm. The measured field of view at a scan angle of ±15° was 1 mm.

The Point Spread Function measurements (below) were carried out by imaging 0.2µm polystyrene fluorescent beads mounted on the surface of a coverslip. From this figure you can see how our unique relay lens system enables having comparable resolution across the field of view.

HyperScope PSF performance measures
PSF measurements taken at the centre and four corners of the field of view

The relay lenses also provide a constant intensity across the field of view, which can be observed on both the 3D surface plot and the 2D plot profile below.

Field flatness was mapped using a homogeneous fluorescent sample. Please note that the field of view of 1 mm corresponds only to the top portion of the plot.

In vivo 6DPF zebrafish larvae expressing GCamp6 imaged using the HyperScope.

Two-photon fixed cell images using the HyperScope

From left to right: Bovine pulmonary artery endothelial cells labelled with MitoTracker® Red CMXRos for mitochondia, stained with Alexa Fluor® 488 phalloidin for F-actin; Muntjac skin fibroblast, F-actin was labelled with green-fluorescent Alexa Fluor® 488 phalloidin; Mouse kidney section stained with Alexa Fluor® 488 wheat germ agglutin - the filamentous actin prevalent in glomeruli and the brush border were stained with red-fluorescent Alexa Fluor® 568.


SciScan is Scientifica's award-winning two-photon acquisition software designed and built by Scientifica in collaboration with researchers.

Scientifica’s multiphoton hardware is also fully integrated into Vidriotech's ScanImage software packages. This software is developed by researchers at the HHMI Janelia Farm research campus specifically for neuroscience applications.


Download the full HyperScope brochure

In vitro setup

HyperScope: In vitro setup

In vivo setup

HyperScope: In vivo setup

Movable in vivo setup

HyperScope: Movable in vivo setup

Extended movable in vivo setup

HyperScope: Extended movable in vivo setup


"The HyperScope is nothing short of remarkable. It is amazing how flexible this scope is and how many things one can do. You can design stimulations and generate various combinations of images. The ability to have two beams and visualise multiple areas of interest, along with the combination of resonant and galvo scanners is what makes this scope distinct from the rest."
Dr. Chi-Hon Lee
"It was great. We were imaging the optic tectum of zebrafish larvae and it was really fast. It’s a really nice system."
Dr. Andrew Boyce, University of Calgary (User at the 2018 Neurophotonics Summer School)
I chose Scientifica’s HyperScope mostly for the modular design. This is a system that is really built for individuals who are wanting to incorporate imaging into electrophysiology. I love the idea that it can handle the second scan path for simultaneous photostimulation. The modular design really allows us to incorporate our slice work.
Dr. Matthew Parsons, The Memorial University of Newfoundland
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