Scientifica HyperScope | Multiphoton Microscopy and Photostimulation
Scientifica HyperScope
Scientifica HyperScope
Scientifica HyperScope
Scientifica HyperScope

Scientifica HyperScope

Push the boundaries of light microscopy and find the answers to the most intricate biological questions with the HyperScope, a proven multiphoton microscope.

Image using two- and three-photon fluorescence excitation with exceptional performance. The HyperScope multiphoton imaging system is a trusted solution that can be customised and upgraded to suit your changing needs. The option of a dual scan head allowing for simultaneous imaging and photostimulation enables you to probe the functional roles of neural cells and circuits.

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Product benefits

Acquire high contrast images of the most intricate samples

The high resolution of the HyperScope reduces background noise, generating accurate data that you can draw reliable conclusions from.

Visualise rapid changes

Use 8kHz fast resonant scanning to capture rapid changes in fluorescent signals. The fast frame rate will enable you to image with the latest high-speed indicators to visualise rapid changes, such as network interactions, and acquire novel data.

Image deep into tissue

Obtain outstanding images of the finest structures deep within your sample. Thanks to multiphoton optical sectioning, stunning three-dimensional reconstructions can be easily produced.

Simultaneous imaging and photostimulation

Full flexibility for integrating wavelengths for both imaging and stimulation. The dual scan head with two light paths enables simultaneous multiphoton imaging and photostimulation with exceptional performance, so you can elucidate the functions of brain cells and circuits.

Proven three-photon capability

Perform much deeper, clearer imaging in vivo and gather more useable data from your samples with the HyperScope. Perform efficient longer wavelength imaging to gather cleaner data at depth, with no further modifications required.

Easily Upgradeable

This flexible system allows you to upgrade and add functionalities as your experiments progress, your funding develops, and your lab grows. Full training and support are provided when you upgrade, so you can get the most out of your equipment.

Simple integration with other techniques

The pioneering slim design means this compact multiphoton system can be easily integrated with other techniques, such as electrophysiology.

All-in-one solution for in vivo and in vitro experiments

All of your experiments can be performed on one system, saving you time and money, and enabling you to acquire data faster and publish sooner.

​Tailor your HyperScope to suit your needs and budget

Available with a variety of options for scan head, frame and detector, the HyperScope can be tailored to suit your needs. Existing components in your lab can also be used to build a HyperScope multiphoton imaging system within your budget. This ensures your research is not limited by your funding.

With easily removable covers, the light paths are accessible for customisation. When in the single scanhead configuration, the second mounting position offers a standardised optomechanical interface where visual stimulus and other custom scan paths can be added.

The ability of the HyperScope to combine imaging with other techniques, such as electrophysiology, means your experiments have no limit.

An all-in-one solution for imaging and stimulation

Push the boundaries of light microscopy with the HyperScope, the ideal solution for using the following techniques to investigate biology:

  • In vivo and in vitro two-photon imaging
  • Three-photon imaging
  • Second and third harmonic generation
  • Two-photon photostimulation
  • Fluorescence lifetime imaging
  • All-optical interrogation of neurons with the HoloStim-3D.

A trusted solution for in vivo and in vitro two-photon imaging

Obtain images from both in vivo and in vitro samples with high spatial resolution to answer novel biological questions. The flexibility of the HyperScope enables it to be easily adapted to suit your experimental requirements, ensuring the raw data is high quality, so less processing and analysis is required.

With the option of a tiltable objective, you can retain optimal alignment during in vivo experiments, without having to tilt the animal. Easily switch between a tiltable objective and regular objective mount, for optimal and cost-effective experimental flexibility.

Imaged during the Drosophila course at Cold Spring Harbor in 2017

Z-stacks from in vivo 6 days post fertilisation zebrafish

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Two-photon recording from a three week old zebrafish brain explant of the line Tg(elavl3:GCaMP6s-nuclear). Four consecutive planes (upper to lower ones from left to right) of a volumetric recording (8 planes in total) are shown where first Dm (amygdala homolog in zebrafish) then Dl (Hippocampus homolog in zebrafish) were electrically stimulated by a glass-electrode (indicated in the upper left corner). Data acquired by Anna Ostenrath and Ewelina Bartoszek, PhD Students in the lab of Professor Emre Yaksi, Kavli Institute, NTNU.

From left to right: Bovine pulmonary artery endothelial cells labelled with MitoTracker® Red CMXRos for mitochondia, stained with Alexa Fluor® 488 phalloidin for F-actin; Muntjac skin fibroblast, F-actin was labelled with green-fluorescent Alexa Fluor® 488 phalloidin; Mouse kidney section stained with Alexa Fluor® 488 wheat germ agglutin - the filamentous actin prevalent in glomeruli and the brush border were stained with red-fluorescent Alexa Fluor® 568.

The underside of an American Oak leaf, imaged using two-photon excitation. Single focus plane from a 3D image projection of the entire imaged volume. Captured using Scientifica's Hyperscope system at Cold Spring Harbour in 2017.

Stereo image of intact ex vivo central nervous system of a Drosophila melanogaster larva with GCaMP6m expressed in all motor neurons. Image acquired on a Hyperscope with James Macleod, with support from Prof. Stefan Pulver and CSHL.

Three-photon imaging: For clearer imaging at depth

Perform much deeper, clearer imaging into tissue and through scattering layers compared to two-photon imaging, which can lose signal and contrast at depth. The HyperScope is optimised for three-photon imaging across the full 900-1700nm wavelength range.

With three-photon imaging, you can acquire cleaner data at depth, see more cells and image through thin scattering layers. Imaging through superficial layers is possible with a reduced need for invasive surgeries.

The HyperScope optics are available in coatings covering the full multiphoton imaging range: from the standard 700 - 1400nm, and now also the 900 - 1700 nm range that is increasingly popular for in vivo experiments.

3D projection of a mouse olfactory bulb, showing GFP labelling of the olfactory glomeruli and sulforhodamin labelling of the blood vessel network. Data kindly provided by Dr. Tobias Ackels at The Francis Crick Institute using our HyperScope and MDU XL during a three-photon demo.

3P images of nestin-positive cells in the ex-vivo sternum, 200 microns deep using the HyperScope. Red: SHG from the bone structure, Green: 3P excited GFP in nestin positive cells.

Three-photon imaging of interneurons in the mouse neocortex. Neurons are labelled with tdTomato expressed under the VGAT promotor. An imaging depth of 1000 microns was achieved, with the dura left intact.

Mitral cells of the mouse olfactory bulb. Left is with dura and right is without. Top: taken using two-photon imaging. Bottom: imaged with three photons, The three-photon images have a higher contrast and show finer details.

Blood vessels in mouse neocortex labelled with fluorescein. Acquired at 1100um depth using three-photon excitation on Scientifica’s Multiphoton microscope, using an Amplitude Mango SP for excitation. Data kindly provided by Dr Adam Packer, University of Oxford.

The mouse neocortex labelled with CellTracker green. Acquired at 1100um depth using three-photon excitation on Scientifica’s Multiphoton microscope, using an Amplitude Mango SP for excitation. Data kindly provided by Dr Adam Packer, University of Oxford.

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Olfactory bulb of a transgenic mouse expressing GCaMP6f in most neurons. Imaged using three-photon excitation. Imaged through the intact dura, through an acute craniotomy.

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This fantastic video shows three-photon imaging of neural activity dynamics in mouse neocortical neurons expressing GCaMP6s 1 mm deep in vivo. Data courtesy of Huriye Atilgan and Adam Packer, University of Oxford.

Scientifica services for this product

Free Lab Consultations

Your Scientifica specialist can help optimise your lab through a free lab consultation, providing advice on the equipment best suited to your planned experiments and lab space.

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Installation & Technical Support

Our installation team can install your system and provide your lab with bespoke training, ensuring your equipment is up and running as soon as possible.

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Scientifica offer product demonstrations, allowing you to ask questions, learn about the multiphoton system and its applications, and see it for yourself.

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Our range of modular parts enable us to tailor our systems to suit your unique research. These can be customised at the point of purchase or later in the lab.

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Lab Move Service

Our lab move service includes the pack down and reinstallation of your electrophysiology or multiphoton imaging system.

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Warranty & Exchange Programme

Scientifica offer a two-year warranty with all Scientifica manufactured goods and a one-year warranty for third party supplied goods. Giving you peace of mind when purchasing your Scientifica equipment.

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Perform all-optical interrogation of neural networks

Seamlessly upgrade the HyperScope with the Scientifica HoloStim-3D to create the industry-leading Spatial Light Modulator (SLM) system. This enables simultaneous two-photon imaging and photostimulation of hundreds of individual cells in 3D. This manipulation of neural activity means the functions of neuronal circuits and subtypes can be explored.


Scientifica’s multiphoton hardware is also fully integrated into Vidriotech's ScanImage software packages. Originally developed at Cold Spring Harbour Laboratories and the HHMI Janelia Research Campus, this software is continually developed to add and enhance features for data collection, specifically for neuroscience applications.

The HyperScope is compatible with other custom software packages, due to the use of industry standard interfaces.


"Working with the Scientifica HyperScope was a great experience, allowing us to perform complex experiments such as dual colour imaging and optogenetics stimulations during imaging sessions"

Dr Mattia Aime
University of Bern

"The HyperScope is nothing short of remarkable. It is amazing how flexible this scope is and how many things one can do. You can design stimulations and generate various combinations of images. The ability to have two beams and visualise multiple areas of interest, along with the combination of resonant and galvo scanners is what makes this scope distinct from the rest."

Dr. Chi-Hon Lee
Academia Sinica, Taiwan

"It was great. We were imaging the optic tectum of zebrafish larvae and it was really fast. It’s a really nice system."

Dr. Andrew Boyce
University of Calgary (User at the 2018 Neurophotonics Summer School)

"I chose Scientifica’s HyperScope mostly for the modular design. This is a system that is really built for individuals who are wanting to incorporate imaging into electrophysiology. I love the idea that it can handle the second scan path for simultaneous photostimulation. The modular design really allows us to incorporate our slice work."

Dr. Matthew Parsons
The Memorial University of Newfoundland

"I have been using the Hyperscope two-photon system for in vivo calcium imaging in behaving mice. The system has been working seamlessly from day one and is very robust, which is especially important in a multi-user environment like my lab."

Sung Eun "Samuel" Kwon, Ph.D.
University of Michigan

"Performing Ca2+ recordings of visual responses in awake mice always require to have extra room under the microscope to set up a treadmill and visual stimulation arrangements. In our case Scientifica HyperScope was very convenient in that regard and currently it is our in vivo imaging workhorse. I also like HyperScope's simplicity, reliability and easy access features which saved us a lot of time."

Dmitry Molotkov, Ph.D.

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