The HoloStim-3D seamlessly integrates with the HyperScope, an award-winning multiphoton imaging system, to create an industry-leading spatial light modulator (SLM) system for all-optical interrogation of neural networks with previously unachievable performance.
Perform two-photon imaging, three-photon imaging, photostimulation and fluorescence lifetime imaging with the HyperScope multiphoton system.
Available with a variety of scanning configurations, the SliceScope or VivoScope frames and a diverse range of detectors, this is a versatile and flexible system that can be tailored and upgraded to suit your experimental needs.
This modular system is now upgradeable to a spatial light modulator (SLM) system for all-optical interrogation of neural networks, with the seamless integration of the HoloStim-3D.
The HyperScope is available as a single or dual scan head with galvo-galvo, resonant-galvo or galvo-galvo-resonant scan mirror configurations. Detectors available include the MDU, MDU XL, ChromoFlex and FLIM Upgrade kit. Take a look at our buyer’s guide to see our full multiphoton range and all scan head, frame and detector options available.
(in vivo or in vitro)
- Three-photon imaging
- Two-photon imaging
- Second harmonic generation
- Two-photon photostimulation
- Fluorescence lifetime imaging
- All-optical interrogation of neurons
Dual scan head
Two light paths enable simultaneous multiphoton imaging and photostimulation with exceptional performance.
Various scanning arrangements
The scan mirrors on the imaging path can be configured as a galvo-galvo, resonant-galvo or galvo-galvo-resonant scan head.
Extended wavelength range
The HyperScope optics are coated for wavelengths of 1300 nm and beyond to give you more experimental flexibility, with proven three-photon capability right off the shelf.
Image up to 4 colours
Compatible with our ChromoFlex detection module to enable multiphoton imaging of up to four colours with highly-sensitive GaAsP and gated GaAsP photomultiplier tubes.
Outstanding images of the finest structures deep within your sample. Thanks to multiphoton optical sectioning, stunning three-dimensional reconstructions can be easily produced.
Pioneering slim design
The compact footprint and flexible design of the multiphoton system allows easy integration with other techniques such as electrophysiology.
Easy access to the light path
Simply remove the cover of the scan head to gain access to the complete light path.
Optional side port
If only one scanning path is mounted, the second mounting position offers access to a side port with a standardised optomechanical interface, allowing researchers to add a custom scan path.
Upgradeable to other imaging capabilities
Compatible with the Scientifica FLIM Upgrade kit for simultaneous fluorescence intensity and fluorescence lifetime imaging, in up to two colour channels.
Upgrade the HyperScope with the Scientifica HoloStim-3D to create a spatial light modulator (SLM) system for simultaneous two-photon imaging and photostimulation of hundreds of cells in 3D.
HyperScope in vitro configuration 360° view
All-optical interrogation of neural networks Expand
Seamlessly integrate the HoloStim-3D with the HyperScope to create an industry-leading Spatial Light Modulator (SLM) system.
Simultaneously stimulate and image hundreds of individual cells in 3D, to interrogate neuronal circuits and identify the role of neural subtypes in brain circuits and behaviours. Cell bodies and subcellular structures can be specifically targeted, allowing you to obtain accurate data that you can draw conclusions from.
Image deeper with three-photon imaging Expand
The HyperScope can perform three-photon imaging without requiring any modifications to the scan head or relay optics. This allows you to perform deeper imaging, image through thin scattering layers or image less-invasively. See some of the data acquired using the HyperScope and MDU XL here.
Olfactory bulb of a transgenic mouse expressing GCaMP6f in most neurons. Imaged using three-photon excitation. Imaged through the intact dura, through an acute craniotomy.
In Vivo Two-Photon Images Expand
Video: in vivo 6DPF zebrafish larvae expressing GCamp6 imaged using the HyperScope
Recording of a Purkinje cell, located in Crus1 in the cerebellum of an adult Bl6 mouse. Credit: Elisabeth Meyer, University of Bristol
Two-Photon Cell Images Expand
Performance Measurements Expand
These measurements were taken with two HyperScope systems, employing a Nikon 16X 0.8 NA 3 mm WD objective at 850 nm. The measured field of view at a scan angle of ±15° was 1 mm.
The Point Spread Function measurements (below) were carried out by imaging 0.2µm polystyrene fluorescent beads mounted on the surface of a coverslip. From this figure you can see how our unique relay lens system enables having comparable resolution across the field of view.
The relay lenses also provide a constant intensity across the field of view, which can be observed on both the 3D surface plot and the 2D plot profile below.
SciScan is Scientifica's award-winning two-photon acquisition software designed and built by Scientifica in collaboration with researchers.
Scientifica’s multiphoton hardware is also fully integrated into Vidriotech's ScanImage software packages. This software is developed by researchers at the HHMI Janelia Farm research campus specifically for neuroscience applications.
The HyperScope is also available with other custom software.
In vitro setup
In vivo setup
Movable in vivo setup
Extended movable in vivo setup
Research papers Expand
Zheng, L., Yu, M., Lin, R. et al. Rhythmic light flicker rescues hippocampal low gamma and protects ischemic neurons by enhancing presynaptic plasticity. Nat Commun 11, 3012 (2020). https://doi.org/10.1038/s41467-020-16826-0
Regan, P., Mitchell, S, J., Kim, S-C. et al. Regulation of synapse weakening through interactions of the microtubule associated protein tau with PACSIN1. Journal of Neuroscience (2021). https://doi.org/10.1523/JNEUROSCI.3129-20.2021
Filter Cube Module
A compact, flexible and efficient epifluorescence illuminator, optimised for use with LED light engines. Specifically designed for Scientifica microscopes, the FCM ensures optimal illumination of samples in all possible configurations.