Scientifica HyperScope | Multiphoton Microscopy and Photostimulation
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    The HoloStim-3D seamlessly integrates with the HyperScope, an award-winning multiphoton imaging system, to create an industry-leading spatial light modulator (SLM) system for all-optical interrogation of neural networks with previously unachievable performance.

    Scientifica HyperScope

    Perform two-photon imaging, three-photon imaging, photostimulation and fluorescence lifetime imaging with the HyperScope multiphoton system.

    Available with a variety of scanning configurations, the SliceScope or VivoScope frames and a diverse range of detectors, this is a versatile and flexible system that can be tailored and upgraded to suit your experimental needs.

    This modular system is now upgradeable to a spatial light modulator (SLM) system for all-optical interrogation of neural networks, with the seamless integration of the HoloStim-3D.

    The HyperScope is available as a single or dual scan head with galvo-galvo, resonant-galvo or galvo-galvo-resonant scan mirror configurations. Detectors available include the MDU, MDU XL, ChromoFlex and FLIM Upgrade kit. Take a look at our buyer’s guide to see our full multiphoton range and all scan head, frame and detector options available.

    Multiphoton Imaging Systems Buyer's Guide

    HyperScope Brochure

    (in vivo or in vitro)

    • Three-photon imaging
    • Two-photon imaging
    • Second harmonic generation
    • Two-photon photostimulation
    • Fluorescence lifetime imaging
    • All-optical interrogation of neurons

    Dual scan head

    Two light paths enable simultaneous multiphoton imaging and photostimulation with exceptional performance.

    Various scanning arrangements

    The scan mirrors on the imaging path can be configured as a galvo-galvo, resonant-galvo or galvo-galvo-resonant scan head.

    Extended wavelength range

    The HyperScope optics are coated for wavelengths of 1300 nm and beyond to give you more experimental flexibility, with proven three-photon capability right off the shelf.

    Image up to 4 colours

    Compatible with our ChromoFlex detection module to enable multiphoton imaging of up to four colours with highly-sensitive GaAsP and gated GaAsP photomultiplier tubes.

    Deep imaging

    Outstanding images of the finest structures deep within your sample. Thanks to multiphoton optical sectioning, stunning three-dimensional reconstructions can be easily produced.

    Pioneering slim design

    The compact footprint and flexible design of the multiphoton system allows easy integration with other techniques such as electrophysiology.

    Easy access to the light path

    Simply remove the cover of the scan head to gain access to the complete light path.

    Optional side port

    If only one scanning path is mounted, the second mounting position offers access to a side port with a standardised optomechanical interface, allowing researchers to add a custom scan path.

    Upgradeable to other imaging capabilities

    Compatible with the Scientifica FLIM Upgrade kit for simultaneous fluorescence intensity and fluorescence lifetime imaging, in up to two colour channels.

    Upgrade the HyperScope with the Scientifica HoloStim-3D to create a spatial light modulator (SLM) system for simultaneous two-photon imaging and photostimulation of hundreds of cells in 3D.

    HyperScope in vitro configuration 360° view

    Seamlessly integrate the HoloStim-3D with the HyperScope to create an industry-leading Spatial Light Modulator (SLM) system.

    Simultaneously stimulate and image hundreds of individual cells in 3D, to interrogate neuronal circuits and identify the role of neural subtypes in brain circuits and behaviours. Cell bodies and subcellular structures can be specifically targeted, allowing you to obtain accurate data that you can draw conclusions from.

    The HyperScope can perform three-photon imaging without requiring any modifications to the scan head or relay optics. This allows you to perform deeper imaging, image through thin scattering layers or image less-invasively. See some of the data acquired using the HyperScope and MDU XL here.


    3D projection of a mouse olfactory bulb, showing GFP labelling of the olfactory glomeruli and sulforhodamin labelling of the blood vessel network. Data kindly provided by Dr. Tobias Ackels at The Francis Crick Institute using our HyperScope and MDU XL during a three-photon demo.
    3P images of nestin-positive cells in the ex-vivo sternum, 200 microns deep. Red: SHG from the bone structure, Green: 3P excited GFP in nestin positive cells.
    3P images of nestin-positive cells in the ex-vivo sternum, 200 microns deep using the HyperScope. Red: SHG from the bone structure, Green: 3P excited GFP in nestin positive cells.
    Mitral cells of the mouse olfactory bulb. Left is with dura and right is without. Top: taken using two-photon imaging. Bottom: imaged with three photons, The three-photon images have a higher contrast and show finer details.


    Olfactory bulb of a transgenic mouse expressing GCaMP6f in most neurons. Imaged using three-photon excitation. Imaged through the intact dura, through an acute craniotomy.

    Video: in vivo 6DPF zebrafish larvae expressing GCamp6 imaged using the HyperScope

    Imaged during the Drosophila course at Cold Spring Harbor in 2017
    Imaged during the Drosophila course at Cold Spring Harbor in 2017
    Z-stacks from in vivo 6 days post fertilisation zebrafish
    Z-stacks from in vivo 6 days post fertilisation zebrafish

    Recording of a Purkinje cell, located in Crus1 in the cerebellum of an adult Bl6 mouse. Credit: Elisabeth Meyer, University of Bristol


    Two-photon fixed cell images using the HyperScope

    From left to right: Bovine pulmonary artery endothelial cells labelled with MitoTracker® Red CMXRos for mitochondia, stained with Alexa Fluor® 488 phalloidin for F-actin; Muntjac skin fibroblast, F-actin was labelled with green-fluorescent Alexa Fluor® 488 phalloidin; Mouse kidney section stained with Alexa Fluor® 488 wheat germ agglutin - the filamentous actin prevalent in glomeruli and the brush border were stained with red-fluorescent Alexa Fluor® 568.

    Above are two images, which were captured using Scientifica's Hyperscope system at Cold Spring Harbour in 2017. The underside of an American Oak leaf was imaged using two-photon excitation.Left: 3D projection of the entire imaged volume. Right: Single focus plane from the same 3D image stack.
    Above are two images, which were captured using Scientifica's Hyperscope system at Cold Spring Harbour in 2017. The underside of an American Oak leaf was imaged using two-photon excitation. Left: 3D projection of the entire imaged volume. Right: Single focus plane from the same 3D image stack.

    These measurements were taken with two HyperScope systems, employing a Nikon 16X 0.8 NA 3 mm WD objective at 850 nm. The measured field of view at a scan angle of ±15° was 1 mm.

    The Point Spread Function measurements (below) were carried out by imaging 0.2µm polystyrene fluorescent beads mounted on the surface of a coverslip. From this figure you can see how our unique relay lens system enables having comparable resolution across the field of view.

    HyperScope PSF performance measures
    PSF measurements taken at the centre and four corners of the field of view

    The relay lenses also provide a constant intensity across the field of view, which can be observed on both the 3D surface plot and the 2D plot profile below.

    Field flatness was mapped using a homogeneous fluorescent sample. Please note that the field of view of 1 mm corresponds only to the top portion of the plot.


    Software

    SciScan is Scientifica's award-winning two-photon acquisition software designed and built by Scientifica in collaboration with researchers.

    Scientifica’s multiphoton hardware is also fully integrated into Vidriotech's ScanImage software packages. This software is developed by researchers at the HHMI Janelia Farm research campus specifically for neuroscience applications.

    The HyperScope is also available with other custom software.

    In vitro setup

    HyperScope: In vitro setup

    In vivo setup

    HyperScope: In vivo setup


    Movable in vivo setup

    HyperScope: Movable in vivo setup

    Extended movable in vivo setup

    HyperScope: Extended movable in vivo setup

    Zheng, L., Yu, M., Lin, R. et al. Rhythmic light flicker rescues hippocampal low gamma and protects ischemic neurons by enhancing presynaptic plasticity. Nat Commun 11, 3012 (2020). https://doi.org/10.1038/s41467-020-16826-0

    Regan, P., Mitchell, S, J., Kim, S-C. et al. Regulation of synapse weakening through interactions of the microtubule associated protein tau with PACSIN1. Journal of Neuroscience (2021). https://doi.org/10.1523/JNEUROSCI.3129-20.2021

    Testimonials

    "The HyperScope is nothing short of remarkable. It is amazing how flexible this scope is and how many things one can do. You can design stimulations and generate various combinations of images. The ability to have two beams and visualise multiple areas of interest, along with the combination of resonant and galvo scanners is what makes this scope distinct from the rest."
    Dr. Chi-Hon Lee
    "It was great. We were imaging the optic tectum of zebrafish larvae and it was really fast. It’s a really nice system."
    Dr. Andrew Boyce, University of Calgary (User at the 2018 Neurophotonics Summer School)
    "I chose Scientifica’s HyperScope mostly for the modular design. This is a system that is really built for individuals who are wanting to incorporate imaging into electrophysiology. I love the idea that it can handle the second scan path for simultaneous photostimulation. The modular design really allows us to incorporate our slice work. "
    Dr. Matthew Parsons, The Memorial University of Newfoundland
    "I have been using the Hyperscope two-photon system for in vivo calcium imaging in behaving mice. The system has been working seamlessly from day one and is very robust, which is especially important in a multi-user environment like my lab."
    Sung Eun "Samuel" Kwon, Ph.D., University of Michigan

    Accessories

    Filter Cube Module

    A compact, flexible and efficient epifluorescence illuminator, optimised for use with LED light engines. Specifically designed for Scientifica microscopes, the FCM ensures optimal illumination of samples in all possible configurations.

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