Simultaneously image and photostimulate hundreds of individual cells in 3D, with precise targeting of cells across large areas of the brain. Introducing the HoloStim-3D.
Interrogate larger neural networks at higher resolution than ever before, with the world's highest performing multiphoton all-optical solution.
The HoloStim-3D seamlessly integrates with the HyperScope, an award-winning multiphoton imaging system, to create an industry-leading spatial light modulator (SLM) system for all-optical interrogation of neural networks with previously unachievable performance.
Investigate neuronal circuits with 3D precision
The HoloStim-3D enables you to perform more complex photostimulation experiments than those that are possible with random-access two-photon or widefield photostimulation.
Discover the roles of neuronal subtypes and brain circuits by simultaneously targeting multiple cells with cellular resolution.
You can observe and record comprehensive network activity by combining precise cell stimulation and imaging with the HoloStim-3D.
By selectively photostimulating only the target cells and rapidly switching between stimulation patterns, you can better understand neuronal interactions in networks.
Selective and accurate targeting
The industry-leading resolution of the Holostim-3D allows cell bodies and subcellular structures to be specifically targeted with minimal off-target activation. This gives you cleaner, more accurate data that you can draw conclusions from.
Analyse larger neuronal networks
Target cells in larger brain regions to understand larger neuronal networks. The large field-of-view of the HoloStim-3D also allows you to use a higher magnification and resolution objective and still achieve the required field-of-view.
Freely combine different imaging and stimulation wavelengths
Being able to excite or image target cells expressing different opsins or fluorescent proteins increases your experimental flexibility.
Perform simultaneous two-photon imaging and photostimulation
Activate and image hundreds of individual cells simultaneously to interrogate neuronal circuits and identify the role of neural subtypes in brain circuits and behaviours.
Deeper imaging and stimulation
Specifically target cells and subcellular structures in layer 4 and beyond, achieving high-quality images.
Mimic neuronal firing
You can quickly switch stimulation patterns to better mimic neuronal firing, improving the accuracy of your data.
High laser transmission
Keep your costs to a minimum by activating the same number of cells with a lower power laser.
The modular design means the HoloStim-3D is seamlessly integrated with the Scientifica HyperScope, a proven multiphoton imaging system, so you can easily upgrade to SLM capabilities and start generating high-quality data faster.
Mouse layer ii/iii neurons expressing GCaMP6s (calcium indicator) and C1V1 (red-shifted channelrhodopsin) are simultaneously stimulated using the HoloStim-3D, while their calcium responses are imaged using resonance two-photon imaging on the HyperScope. In the total 100 cells were stimulated at 6 nJ per cell, using a spiral pattern. Data acquired by Kelly Sakaki (Scientifica), Robert Lees and Adam Packer (both University of Oxford).
HoloStim-3D on the HyperScope 360° view
Adopting SLM technology is made easier with full integration of globally-recognised ScanImage software, saving you time so you can start experimenting faster.
Design & Specifications
|Wavelength range standard||
Wavelength range standard920 - 1064 nm
|Wavelength range optional||
Wavelength range optional700 - 1300 nm
|Stimulation point resolution||
Stimulation point resolution<2.5 microns
Array size17.7 x 10.6 mm
|SLM response time||
SLM response time3.3 ms
|SLM array resolution||
SLM array resolution1920 x 1152 pixels