Scientifica VivoScope | Multiphoton Imaging System
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    Scientifica VivoScope

    The VivoScope is a slimline upright microscope. When combined with a multiphoton scan head, it forms the foundation of the finest in vivo imaging system for two-photon behavioural studies.

    The VivoScope is ideal for larger in vivo samples, linear or spherical treadmills, large stereotaxic frames or other virtual reality set-ups. The extended position of the scan head gives more room between the light path on your sample and the microscope frame.

    Benefits

    Extra space

    The extended scan head gives more room between the light path onto your sample and the microscope frame without compromising stability (205.94 mm up from 130 mm).

    Galvo or Resonant

    The VivoScope is available with both galvo and resonant scan head options, depending on your experimental requirements.

    Acoustic isolation

    Our custom scan head enclosure integrates acoustic insulating foam to dramatically reduce the audible noise created by the resonant motor without affecting performance.

    Patch under multiphoton illumination

    The fast frame rate provides the visual feedback required to position your patch pipette accurately under infrared laser light.

    Deep imaging for large populations of cells

    Tailored to large back aperture objectives, the entire field of view can be quickly scanned to monitor the activity from a large number of cells.

    The VivoScope microscope frame is available with resonant or galvo scan heads and a variety of detectors including the MDU, MDU XL and FLIM Upgrade kit. Take a look at our buyer’s guide to see our full multiphoton range and all scan head, frame and detector options available.

    The VivoScope in action at the Allen Institute

    The Allen Institute for Brain Science is using three VivoScopes to collect data for the Allen Brain Observatory, an ongoing project to visualise the brain in action. Watch the video below for more information:


    Control options

    The VivoScope can be driven by Vidrio Technologies ScanImage or custom software.

    Design & Specifications

    Scanning mirror (y-galvo)
    Scanning mirror (y-galvo)
    3 x 5 mm 8315KL scanning mirror (MicroMax 671HP driver)
    Scanning mirror (x-galvo)
    Scanning mirror (x-galvo)
    2 kHz in bidirectional scan mode (4 fps @ 512x512 px), 1 kHz in unidirectional scanning mode (2 fps @ 512x512 px)
    Scanning mirror (x-resonant)
    Scanning mirror (x-resonant)
    3 x 5 mm 8 kHz CRS series scanner from Cambridge Technologies (15 fps @ 1024 x 1024 px, 30 fps @ 512 x 512 px, 60 fps @ 512 x 256 px)
    Beam input height
    Beam input height
    280.6 mm
    Input beam diameter
    Input beam diameter
    <3 mm
    Beam diameter at back aperture
    Beam diameter at back aperture
    <20.1 mm
    Beam expansion
    Beam expansion
    6.7x
    Relay lens expansion
    Relay lens expansion
    1x
    Lens coating
    Lens coating
    700-1200 nm (Rav <0.5%)
    Maximum scan angles (galvo)
    Maximum scan angles (galvo)
    +/- 10 optical degrees
    Maximum scan angles (scan head)
    Maximum scan angles (scan head)
    +/- 7.6 optical degrees
    Galvo voltage/optical degree
    Galvo voltage/optical degree
    0.25 V
    Turning mirror size
    Turning mirror size
    45 x 64 x 6 mm
    Turning mirror coating
    Turning mirror coating
    Protected Silver (98% reflectivity)
    Typical field of view
    Typical field of view
    ~600 µm2 (16x), ~500 µm2 (20x)
    Scan control
    Scan control
    ScanImage 5

    VivoScope Multiphoton Microscope SchematicVivoScope Multiphoton Microscope Schematic

    VivoScope Multiphoton Microscope Schematic

    VivoScope moving microscope with a Tiltable Objective Mount (TOM) and 100um Piezo

    Erskine, A., Ackels, T., Dasgupta, D., Fukunaga, I., Schaefer, A. (2019). Mammalian olfaction is a high temporal bandwidth sense. bioRxiv https://www.biorxiv.org/content/biorxiv/early/2019/03/09/570689.full.pdf

    Roland, B., Deneux, T., Franks, K., Bathellier, B. and Fleischmann, A. (2018). Odor identity coding by distributed ensembles of neurons in the mouse olfactory cortex. eLIFE http://dx.doi.org/10.7554/eLif...

    Sun, B., Wang, M., Hoerder-Suabedissen, A., Xu, C., Packer, A., & Szele, F. (2022). Intravital Imaging of the Murine Subventricular Zone with Three Photon Microscopy. Cerebral Cortex. https://doi.org/10.1093/cercor/bhab400

    Sun, L., Chen, R., Bai, Y., Li, J., Wu, Q., Shen, Q., Wang, X. (2018). Morphological and Physiological Characteristics of Ebf2-EGFP-Expressing Cajal-Retzius Cells in Developing Mouse Neocortex. Cerebral Cortex https://doi.org/10.1093/cercor/bhy265

    Garrett, M., Manavi, S., Roll, K., Ollerenshaw, D., Groblewski, P., Ponvert, N., Kiggins, J., Casal, L., Mace, K., Williford, A., Leon, A., Jia, X., Ledochowitsch, P., Buice, M., Wakeman, W., Mihalas, S. and Olsen, S. (2020). Experience shapes activity dynamics and stimulus coding of VIP inhibitory cells. eLife, https://elifesciences.org/articles/50340

    Umpierre, A., Bystrom, L., Ying, Y., Liu, Y., Worrell, G. and Wu, LJ. (2020). Microglial calcium signaling is attuned to neuronal activity in awake mice. eLife, https://elifesciences.org/articles/56502

    Accessories

    Movable Periscope Bracket (MP-4010-30)

    This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

    Filter Cube Module

    A compact, flexible and efficient epifluorescence illuminator, optimised for use with LED light engines. Specifically designed for Scientifica microscopes, the FCM ensures optimal illumination of samples in all possible configurations.

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