The HoloStim-3D seamlessly integrates with the HyperScope, an award-winning multiphoton imaging system, to create an industry-leading spatial light modulator (SLM) system for all-optical interrogation of neural networks with previously unachievable performance.
The VivoScope is a slimline upright microscope. When combined with a multiphoton scan head, it forms the foundation of the finest in vivo imaging system for two-photon behavioural studies.
The VivoScope is ideal for larger in vivo samples, linear or spherical treadmills, large stereotaxic frames or other virtual reality set-ups. The extended position of the scan head gives more room between the light path on your sample and the microscope frame.
The VivoScope microscope frame is available with resonant or galvo scan heads and a variety of detectors including the MDU, MDU XL, ChromoFlex and FLIM Upgrade kit. Take a look at our buyer’s guide to see our full multiphoton range and all scan head, frame and detector options available.
The VivoScope in action at the Allen Institute
The Allen Institute for Brain Science is using three VivoScopes to collect data for the Allen Brain Observatory, an ongoing project to visualise the brain in action. Watch the video below for more information:
Design & Specifications
|Scanning mirror (y-galvo)||
Scanning mirror (y-galvo)3 x 5 mm 8315KL scanning mirror (MicroMax 671HP driver)
|Scanning mirror (x-galvo)||
Scanning mirror (x-galvo)2 kHz in bidirectional scan mode (4 fps @ 512x512 px), 1 kHz in unidirectional scanning mode (2 fps @ 512x512 px)
|Scanning mirror (x-resonant)||
Scanning mirror (x-resonant)3 x 5 mm 8 kHz CRS series scanner from Cambridge Technologies (15 fps @ 1024 x 1024 px, 30 fps @ 512 x 512 px, 60 fps @ 512 x 256 px)
|Beam input height||
Beam input height280.6 mm
|Input beam diameter||
Input beam diameter<3 mm
|Beam diameter at back aperture||
Beam diameter at back aperture<20.1 mm
|Relay lens expansion||
Relay lens expansion1x
Lens coating700-1200 nm (Rav <0.5%)
|Maximum scan angles (galvo)||
Maximum scan angles (galvo)+/- 10 optical degrees
|Maximum scan angles (scan head)||
Maximum scan angles (scan head)+/- 7.6 optical degrees
|Galvo voltage/optical degree||
Galvo voltage/optical degree0.25 V
|Turning mirror size||
Turning mirror size45 x 64 x 6 mm
|Turning mirror coating||
Turning mirror coatingProtected Silver (98% reflectivity)
|Typical field of view||
Typical field of view~600 µm2 (16x), ~500 µm2 (20x)
Scan controlSciScan, ScanImage 5
VivoScope moving microscope with a Tiltable Objective Mount (TOM) and 100um Piezo
Research Papers Expand
Erskine, A., Ackels, T., Dasgupta, D., Fukunaga, I., Schaefer, A. (2019). Mammalian olfaction is a high temporal bandwidth sense. bioRxiv https://www.biorxiv.org/content/biorxiv/early/2019/03/09/570689.full.pdf
Roland, B., Deneux, T., Franks, K., Bathellier, B. and Fleischmann, A. (2018). Odor identity coding by distributed ensembles of neurons in the mouse olfactory cortex. eLIFE http://dx.doi.org/10.7554/eLif...
Sun, L., Chen, R., Bai, Y., Li, J., Wu, Q., Shen, Q., Wang, X. (2018). Morphological and Physiological Characteristics of Ebf2-EGFP-Expressing Cajal-Retzius Cells in Developing Mouse Neocortex. Cerebral Cortex https://doi.org/10.1093/cercor/bhy265
Garrett, M., Manavi, S., Roll, K., Ollerenshaw, D., Groblewski, P., Ponvert, N., Kiggins, J., Casal, L., Mace, K., Williford, A., Leon, A., Jia, X., Ledochowitsch, P., Buice, M., Wakeman, W., Mihalas, S. and Olsen, S. (2020). Experience shapes activity dynamics and stimulus coding of VIP inhibitory cells. eLife, https://elifesciences.org/articles/50340
Umpierre, A., Bystrom, L., Ying, Y., Liu, Y., Worrell, G. and Wu, LJ. (2020). Microglial calcium signaling is attuned to neuronal activity in awake mice. eLife, https://elifesciences.org/articles/56502
Movable Periscope Bracket (MP-4010-30)
This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.