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LED Illumination for In-Vitro Optogenetics and High Speed Fluorescence Microscopy
CoolLED's pE-300ultra offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains. Users have access to both microsecond switching via multiple TTL inputs and the ability to mount inline excitation filters. This, when paired with today’s high performance multi band filter sets, facilitates imaging traditionally done via a white light source and a filter wheel, with all the benefits of LED.
As part of the pE-300 Series, the NEW Enhanced pE-300ultra now delivers DOUBLE the intensity at the microscope sample plane. It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.
The pE-300ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength intensity and shuttering. Until now these benefits have only been accessible to users of high end, highly flexible illuminators such as the CoolLED pE-4000.
A broad spectrum of illumination is provided, covering the excitation bands of common fluorescent stains.
The system comprises a pE-300ultra Light Source, Control Pod, set of three Excitation Filter Holders and Power Supply. It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope. An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.
Multiple TTL Inputs
Triggering from multiple TTL inputs, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.
Ideal for Multi-Band Filter Sets
A benefit of the pE-300ultra is that stains can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether.
An important additional feature of the pE-300ultra is that it includes CoolLED’s “Sequence Runner” multiple channel excitation mode. Users can define the order of their fluorophore capture using their pE-300ultra Control Pod, then the pE-300ultra light source can accept a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the light source. This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.
With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300ultra can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.
Direct-fit for connecting to a microscopes – by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.
Liquid Light Guide with a fixed 3mm diameter, liquid light guide. An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.
Manual: Manual control for instant on/off and intensity control in 1% steps from 0 – 100% Individual bands can be selected/deselected and controlled independently as desired
Remote: Via global and individual channel TTL for on/off control of selected channels using a BNC connection on the light source. Triggering speed <20us at full power
Connectivity: Remote via USB (B type) for imaging software control
Sequence Runner: Single TTL input to step through user defined sequence. Triggering speed <20us at full power
CoolLED provide a 36 month warranty on the system the LEDs.
Power requirements100-240V a.c. 50/60Hz, 1.4A
Power consumptionStandby Max 2W
|1 band (GYR) at 100% intensity Max 38W|
|2 bands (BLU & GYR) at 100% intensity Max 63W|
|White (all 3 bands) at 100% intensity 80W|
DimensionspE-300ultra Light Source: 77mm(w) x 186mm(d) x 162(h) - Weight 1.4kg
|pE-300ultra Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg|
|pE-300ultra Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg|
|pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg|
|Environment & Safety||
Environment & SafetyMercury-free
|Energy Efficient: 80% less power|
|Long lifetime (25,000 operating hours)|
|No bulb replacements|
|Reduced risk of eye damage|
|No special disposal regulations or issues|