CoolLED pE-800fura
Supercharged calcium imaging and beyond
Building on the successful pE-800 Illumination System, the pE-800fura offers the most comprehensive calcium imaging system available, as well as enhancing everyday fluorescence microscopy by combining lightning fast switching with eight individually controllable channels, tailored to the most popular fluorophores and calcium indicators.
Product benefits
True 8-channel illumination
Eight powerful and efficient LEDs offer spectral coverage from 340-635 nm, tailored to fluorophores for everyday fluorescence ranging from DAPI to Cy5.
Individually controlling all eight channels is achieved via USB, TTL or analogue for easily matching each LED to the selected calcium indicator or fluorophore set.
Unlike lasers, the broader spectral output of LEDs can be refined by the use of optical filters in order to excite a range of dyes, expanding the coverage of the pE-800fura. The ability to customise the wavelength output with both wavelength switching and optical filters means experiments are no longer limited by the light source, even as requirements evolve or new dyes emerge in the future.
Offering high-speed wavelength selection across the spectrum, the pE-800fura presents the first direct LED upgrade to traditional monochromator solutions with all the benefits of LED illumination.
An affordable, automated system
When combined with inline filters, the pE-800fura transforms a manual microscope into an affordable and powerful eight-channel automated system for calcium imaging, ratiometric assays and everyday fluorescence.
Protecting against photobleaching and phototoxicity
Industry-leading <7 µs hardware synchronisation protects against photobleaching and phototoxicity by restricting sample exposure to image acquisition. Balancing irradiance to the lowest level possible while still maintaining image quality is another approach for reducing photodamage, and the pE-800fura simplifies this with fine irradiance control from 0-2%, and in 1% steps up to 100%.
Not everyone realises that excitation filters can still leak unwanted light, reducing the signal-to-noise ratio. The ability to select a single LED channel overcomes this, and has the added benefit of allowing a lower concentration of dyes such as Fura-2, which improves cell health and viability due to reduced toxicity – and also reduces reagent cost.
These factors not only improve data quality, but also push the boundaries of time-lapse studies.
Intuitive software control
Software control is achieved through a fast USB 2.0 connection and and support in most major imaging software programs. The user-friendly LightBridge Graphical User Interface (GUI) also features control options including:
- On/off control
- LED selection
- Real time irradiance control
- Sequence Runner
- Save and load pre-sets
- pE-800fura start-up settings
- Analogue settings
Speak to one of our experts for details on pricing, features, installation and support.
