Fluorescence Lifetime Imaging – Another Dimension in Multiphoton Imaging: Watch the live recording here
On 7th July, Dr. Christian Wilms, Research & Development Manager, Scientifica, gave an interesting talk at the Imperial Neurotechnology 2021 – Centre for Neurotech Annual Research Symposium.
Talk Abstract
Commonly, fluorescence microscopy uses the colour and intensity of fluorescence to generate images. A third parameter of fluorescence is less commonly used in imaging: the excited state lifetime of a fluorescent molecule (aka the fluorescence lifetime). Similar to colour, the fluorescence lifetime is an absolute value which is not influenced by properties of the measurement system or by local concentrations of the fluorescent dye. As a result, fluorescence lifetime imaging (FLIM) can provide quantitative insights into the physiology of live cells. In combination with two-photon excitation, it becomes a powerful tool for the quantitative study of cellular signalling in deep tissue. In this talk we provide an introduction to FLIM, including the required instrumentation as well as introducing some example data to demonstrate versatility and potential of the technology for neuroscience researchers.
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