Fluorescence Lifetime Imaging – Another Dimension in Multiphoton Imaging: Watch the live recording here

Fluorescence Lifetime Imaging – Another Dimension in Multiphoton Imaging: Watch the live recording here


On 7th July, Dr. Christian Wilms, Research & Development Manager, Scientifica, gave an interesting talk at the Imperial Neurotechnology 2021 – Centre for Neurotech Annual Research Symposium.

Talk Abstract

Commonly, fluorescence microscopy uses the colour and intensity of fluorescence to generate images. A third parameter of fluorescence is less commonly used in imaging: the excited state lifetime of a fluorescent molecule (aka the fluorescence lifetime). Similar to colour, the fluorescence lifetime is an absolute value which is not influenced by properties of the measurement system or by local concentrations of the fluorescent dye. As a result, fluorescence lifetime imaging (FLIM) can provide quantitative insights into the physiology of live cells. In combination with two-photon excitation, it becomes a powerful tool for the quantitative study of cellular signalling in deep tissue. In this talk we provide an introduction to FLIM, including the required instrumentation as well as introducing some example data to demonstrate versatility and potential of the technology for neuroscience researchers.



Sign up to receive the latest news and resources

Sign up to our news bulletin to receive the latest advice guides, news, events, webinars and product updates. All content is focused around giving you the tools to achieve ground-breaking findings.

Scientifica FLIM Upgrade Kit

The Scientifica FLIM upgrade kit is an integrated and flexible Fluorescence Lifetime Imaging solution. When installed on a Scientifica multiphoton system, the FLIM upgrade enables simultaneous fluorescence intensity and fluorescence lifetime imaging.

Contact Form

At Scientifica we take your privacy seriously and will only use your personal information to provide the products and services you have requested from us. If you consent to us collecting and storing your data from this form please tick the checkbox below. You can withdraw your consent at any time. For more information please view our Privacy Policy.  *

Scientifica provide a variety of content we think you will find useful. This includes information such as events news, latest product updates, a fortnightly news bulletin and advice guides on neuroscience experimental techniques. By choosing to receive communications about our latest news, we may send you information for market research purposes from time to time.

To enable us to ensure you receive communications that are relevant to you, please tell us which of the following areas you are interested in.