Scientifica SciCam Pro Camera
The SciCam Pro is a high resolution CCD camera specifically designed for use with electrophysiology setups. It is ideal for fluorescence imaging, live cell imaging, calcium imaging and electrophysiology.
Built in collaboration with scientific camera experts QImaging, the fanless cooling system and built-in grounding point create zero vibration and electrical noise for careful electrode positioning and fine electrophysiological recordings.
Applications
- Electrophysiology
- Fluorescence microscopy
- Calcium imaging
- Live cell imaging
- Infrared DIC
Software
The SciCam Pro is packaged with QImaging's Ocular software. Designed from the ground up, it is the ideal software choice for image capture in microscopy. The software can be easily installed and enables intuitive image capture.
Learn more about the Ocular software
Merged Widefield IR and Fluorescence Images
Fluorescence images of in vivo 6DPF zebra fish larvae expressing GCamp6, imaged using the SciCam Pro camera
Widefield Fixed Cell Fluorescence Images
Widefield fluorescence images captured using the SciCam Pro camera. From left to right: Bovine Pulmonary Artery Endothelial Cells labelled with AlexaFluor 488 phalloidin for F-actin; Mouse kidney section stained with Alexa Fluor® 488 wheat germ agglutinin; Muntjac skin fibroblast labelled with green fluorescent Alexa Fluor® 488 phalloidin for F-actin.
Design & Specifications
| CCD Sensor | |
|---|---|
| Sensor Type | Sony ICX-825 Scientific Interline CCD (Monochrome) |
| CCD Array | 1360 x 1024 |
| Pixel Size | 6.45μm x 6.45μm |
| Sensor Dimensions | 8.8mm x 6.6mm (11mm diagonal) |
| Peak Quantum Efficiency | 75% at 600nm |
| Full Well Capacity | >11,000e- single pixel |
| Camera | |
| Digital Output | 14-bit with 50MHz readout |
| Digitization Rate | USB3: 50MHz high frame rate |
| Read Noise (typical) | <5.5e- RMS with 50MHz readout |
| Frame Rate | 22 fps (full resolution), 31 fps (binned 2x2) |
| Exposure Time Range | 25µs - 5secs |
| Supported Binning Modes | 1x1, 2x2, 4x4, 6x6, 8x8, 12x12, 16x16 |
| Dark Current Rate (typical) | 0.036 e/p/s at +15ºC regulated |
| Sensor Cooling | 0ºC stabilised at 22ºC ambient, Thermoelectric cooling with convection |
| Intelligent Quantification Features | DPC - Defective Pixel Correction |
| Interfacing | |
| Computer Platforms/Operating Systems | Windows 7 (64 bit), Windows 8 (64 bit), Windows 10 (64 bit). Refer to the QImaging website for the latest list of minimum computer recommendations |
| Digital Interface | USB3.0 (USB2 compatible at reduced max fps) |
| Triggering I/O Signals | Trigger In, Expose Out, End-of-Frame, Shutter Out |
| Supported Triggering Modes | Trigger First, Strobe, Bulb |
| Shutter Modes | Open Never, Open Pre-exposure, Open Pre-sequence, Open Pre-trigger, Open no change |
| Mechanical | |
| Optical Interface | 1”, C-mount optical format |
| Mounting Hole Thread Size | 1/4” - 20 thread, 4 sides |
| Camera Dimensions | 98.4mm x 76mm x 76mm (length x width x height) |
| Weight | 1.55lb, 0.72kg |
| Power Requirement | 7.5V DC, 2.5A |
Schematics
Spectral Response
Research Papers
Annecchino, L., Morris, A., Copeland, C., Agabi, O., Chadderton, P. and Schultz, S. (2018). Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology. Neuron, 95(5), p1048–1055 http://dx.doi.org/10.1016/j.ne...
Booker, S., Sumera, a., Kind, P. and Wyllie, D. (2021). Contribution of N-methyl D-aspartate receptors to synaptic function in rat hippocampal interneurons. eNeuro, https://doi.org/10.1523/ENEURO...
Gelegen, C., Miracca, G., Ran, MZ., Harding, EC., Ye, Z., Yu, X., Tossell, K., Housten, CM., Yustos, R., Hawkins, ED., Vyssotski, AL., Dong, HL., Wisden, W., and Franks, NP. (2018). Excitatory Pathways from the Lateral Habenula Enable Propofol-Induced Sedation. Cell Press, 28(4), p580-587. https://www.sciencedirect.com/...