Scientifica SciCam Pro Camera

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Scientifica SciCam Pro Camera

The SciCam Pro is a high resolution CCD camera specifically designed for use with electrophysiology setups. It is ideal for fluorescence imaging, live cell imaging, calcium imaging and electrophysiology.

Built in collaboration with scientific camera experts QImaging, the fanless cooling system and built-in grounding point create zero vibration and electrical noise for careful electrode positioning and fine electrophysiological recordings.

Benefits

Fanless cooling

Cooling fins create a heat sink to enable fanless cooling for completely vibration-free imaging.

Fast frame rates

50 MHz pixel digitisation delivers fast frame rates for smooth visualisation of electrode positioning in electrophysiology.

Integrated grounding point

Electrically isolate the whole camera with ease.

High sensitivity

75% peak QE enables the detection of weak signals not possible on industrial cameras, making the SciCam Pro ideal for low-light fluorescence imaging and calcium imaging.

Pristine images

Intelligent Quantification algorithms enable Defective Pixel Correction (DPC) to provide flawless, high-quality images.

Regulated sensor cooling

Regulated sensor cooling provides high-quality images for long experiments.

Applications

  • Electrophysiology
  • Fluorescence microscopy
  • Calcium imaging
  • Live cell imaging
  • Infrared DIC

Software

The SciCam Pro is packaged with QImaging's Ocular software. Designed from the ground up, it is the ideal software choice for image capture in microscopy. The software can be easily installed and enables intuitive image capture.

Learn more about the Ocular software

Merged Widefield IR and Fluorescence Images

Fluorescence images of in vivo 6DPF zebra fish larvae expressing GCamp6, imaged using the SciCam Pro camera

Widefield Fixed Cell Fluorescence Images

Widefield fluorescence images captured using the SciCam Pro camera. From left to right: Bovine Pulmonary Artery Endothelial Cells labelled with AlexaFluor 488 phalloidin for F-actin; Mouse kidney section stained with Alexa Fluor® 488 wheat germ agglutinin; Muntjac skin fibroblast labelled with green fluorescent Alexa Fluor® 488 phalloidin for F-actin.

Design & Specifications

CCD Sensor
CCD Sensor
Sensor Type
Sensor Type
Sony ICX-825 Scientific Interline CCD (Monochrome)
CCD Array
CCD Array
1360 x 1024
Pixel Size
Pixel Size
6.45μm x 6.45μm
Sensor Dimensions
Sensor Dimensions
8.8mm x 6.6mm (11mm diagonal)
Peak Quantum Efficiency
Peak Quantum Efficiency
75% at 600nm
Full Well Capacity
Full Well Capacity
>11,000e- single pixel
Camera
Camera
Digital Output
Digital Output
14-bit with 50MHz readout
Digitization Rate
Digitization Rate
USB3: 50MHz high frame rate
Read Noise (typical)
Read Noise (typical)
<5.5e- RMS with 50MHz readout
Frame Rate
Frame Rate
22 fps (full resolution), 31 fps (binned 2x2)
Exposure Time Range
Exposure Time Range
25µs - 5secs
Supported Binning Modes
Supported Binning Modes
1x1, 2x2, 4x4, 6x6, 8x8, 12x12, 16x16
Dark Current Rate (typical)
Dark Current Rate (typical)
0.036 e/p/s at +15ºC regulated
Sensor Cooling
Sensor Cooling
0ºC stabilised at 22ºC ambient, Thermoelectric cooling with convection
Intelligent Quantification Features
Intelligent Quantification Features
DPC - Defective Pixel Correction
Interfacing
Interfacing
Computer Platforms/Operating Systems
Computer Platforms/Operating Systems
Windows 7 (64 bit), Windows 8 (64 bit), Windows 10 (64 bit). Refer to the QImaging website for the latest list of minimum computer recommendations
Digital Interface
Digital Interface
USB3.0 (USB2 compatible at reduced max fps)
Triggering I/O Signals
Triggering I/O Signals
Trigger In, Expose Out, End-of-Frame, Shutter Out
Supported Triggering Modes
Supported Triggering Modes
Trigger First, Strobe, Bulb
Shutter Modes
Shutter Modes
Open Never, Open Pre-exposure, Open Pre-sequence, Open Pre-trigger, Open no change
Mechanical
Mechanical
Optical Interface
Optical Interface
1”, C-mount optical format
Mounting Hole Thread Size
Mounting Hole Thread Size
1/4” - 20 thread, 4 sides
Camera Dimensions
Camera Dimensions
98.4mm x 76mm x 76mm (length x width x height)
Weight
Weight
1.55lb, 0.72kg
Power Requirement
Power Requirement
7.5V DC, 2.5A

Research Papers

Annecchino, L., Morris, A., Copeland, C., Agabi, O., Chadderton, P. and Schultz, S. (2018). Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology. Neuron, 95(5), p1048–1055 http://dx.doi.org/10.1016/j.ne...

Gelegen, C., Miracca, G., Ran, MZ., Harding, EC., Ye, Z., Yu, X., Tossell, K., Housten, CM., Yustos, R., Hawkins, ED., Vyssotski, AL., Dong, HL., Wisden, W., and Franks, NP. (2018). Excitatory Pathways from the Lateral Habenula Enable Propofol-Induced Sedation. Cell Press, 28(4), p580-587. https://www.sciencedirect.com/...

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