Scientifica Multiphoton Detection Unit (MDU)

Design and specifications

Number of channels: Two per MDU
Detector types: Bi- and multi-alkali PMTs, protected and gated GaAsP PMTs
Primary dichroic mirror: 665 nm long-pass dichroic
Laser blocking filter: 680 nm short-pass filter
Maximum objective exit aperture: 24 mm diameter
Angular collection: Collects 5° of divergent light
Objective compatibility: Water-dipping M32X0.75, M27X0.75, M25X0.75 and RMS threaded types (direct or via adapters)
Spectral filtration: One Olympus U-MF2 filter cube supplied (un-populated) compatible with standard fluorescence filter sets (25 mm filters and 24 x 36 mm dichroic mirrors, 1 mm thick)
Preamplifier bandwidth: 1 MHz for galvo detection, 20 MHz for resonant detection. Variable preamplifiers available for fine tuning of bandwidth and gain
Preamplifier gain: 200,000 (galvo detection), 100,000 (resonant detection)
Output connection: SMA-BNC to the preamp and 3m BNC from preamp to DAQ
Inputs: BNC input for gate control on Gated GaAsP variant

Research Papers

Target-Specific expression of presynaptic NMDA receptors in neocortical microcircuits

Buchanan, K.A. et al. (2012) Target-Specific expression of presynaptic NMDA receptors in neocortical microcircuits, Neuron, 75(3), pp. 451–466. https://doi.org/10.1016/j.neur....
Near-Infrared-Induced heating of confined water in polymeric particles for efficient payload release

Viger, M.L. et al. (2014) Near-Infrared-Induced heating of confined water in polymeric particles for efficient payload release, ACS Nano, 8(5), pp. 4815–4826. https://doi.org/10.1021/nn5007....
Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging

Johnston, J. et al. (2014) Rapid mapping of visual receptive fields by filtered back projection: application to multi‐neuronal electrophysiology and imaging, The Journal of Physiology, 592(22), pp. 4839–4854. Available at: https://doi.org/10.1113/jphysi....
Recording Neural Activity in Unrestrained Animals with Three-Dimensional Tracking Two-Photon Microscopy

Karagyozov, D. et al. (2018) Recording Neural Activity in Unrestrained Animals with Three-Dimensional Tracking Two-Photon Microscopy, Cell Reports, 25(5), pp. 1371-1383.e10. Available at: https://doi.org/10.1016/j.celr....