Scientifica Multiphoton Detection Unit (MDU)

We have a variety of multiphoton imaging systems, electrophysiology rigs, manipulators and staging available at a reduced price. Find out more here.

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Scientifica Multiphoton Detection Unit (MDU)

An optimised two-channel fluorescence collection and detection system designed for multiphoton fluorescence imaging.

Designed for integration with Scientifica’s SliceScope microscope, the MDU comprises pre-aligned optics, safety shutters/switches, a filter cube and either one or two photomultiplier tubes (PMTs). Each detection unit also contains a power supply, preamplifier and control unit.

Benefits

Customised optics

A custom designed, extra-large fixed position collection lens is sited very close to the objective back aperture, allowing photon collection at the widest of scan angles

High signal to noise ratio

The electronic design of the MDU performs exceptionally by minimising background noise which can degrade the image quality.

Fast filter exchange

A standard Olympus filter cube is fitted to a dovetail slider which can easily move in and out of the MDU, easing the setup of the next set of experiments.

Increased photon capture

Maximised light collection from a wide range of objectives and condensers. Photons can be harvested from objectives that have either a small or large back apertures (RMS, M25, M27 and M32 thread adapters available).

Above or below the sample

Collect reflected fluorescence through the microscope objective, or transmitted fluorescence using a high-NA oil-immersion condenser lens. Alternatively combine two MDUs simultaneously for optimum signal collection in up to four channels.

Design & Specifications

Laser/visible dichroic mirror
Laser/visible dichroic mirror
685 nm long-pass dichroic
Laser blocking filter
Laser blocking filter
680 nm short-pass filter
Maximum objective exit aperture
Maximum objective exit aperture
24 mm diameter
Angular collection
Angular collection
+/- 5 degrees maximum from 20 mm objective, +/- 3.5 degrees from 24 mm objective
Objective compatibility
Objective compatibility
Water-dipping M32X0.75, M27X0.75, M25X0.75 and RMS threaded types (direct or via adapters)
Spectral filtration
Spectral filtration
One Olympus U-MF2 filter cube supplied (un-populated) compatible with standard fluorescence filter sets (25 mm filters and 24 x 36 mm dichroic mirrors, 1 mm thick)
Number of channels
Number of channels
Two per MDU
Detector types
Detector types
Hamamatsu R9880U-210, R9880U-01, R9880U-20
Preamplifier bandwidth
Preamplifier bandwidth
1.35 MHz (galvo detection), 20 MHz (resonant detection)
Preamplifier gain
Preamplifier gain
200,000 (galvo detection), 100,000 (resonant detection)
Output connection
Output connection
SMA (3.5 m signal leads supplied)

multiphoton detection unit schematicmultiphoton detection unit schematicmultiphoton detection unit schematic

Research Papers

Target-Specific Expression of Presynaptic NMDA Receptors in Neocortical Microcircuits

Neuron

View paper

Near-Infrared-Induced Heating of Confined Water in Polymeric Particles for Efficient Payload Release

ACS Nano

View paper

Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging

The Journal of Physiology

View paper

Recording Neural Activity in Unrestrained Animals with Three-Dimensional Tracking Two-Photon Microscopy

Cell Reports

View paper

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