Simultaneously image and photostimulate hundreds of individual cells in 3D, with precise targeting of cells across large areas of the brain. Introducing the HoloStim-3D.
With improved image quality and easy handling, the Olympus CKX53 delivers stable performance and a more efficient cell culture process for a variety of cell culture needs including live cell observation, cell sampling and handling, image capture, and fluorescence observation.
Fast and Efficient Cell Observation with the Integrated Phase Contrast (iPC) System
The high contrast achieved by the CKX53 iPC system provides a clear view of cells at 4x, 10x, 20x, and 40x without the user having to exchange or re-center the ring slit. The new phase contrast system facilitates simple and efficient cell observation for a faster, more comfortable workflow.
Clear, Wide View with the 2X Objective and FN 22
The ring slit for the PLN2X objective, CKX3-SLPAS, has a 22 mm field of view and an 11 mm diameter. As a result, observation using the objective is perfect for efficiently screening desired cells, enabling a faster cell culture process. The 2X objective provides noticeably higher contrast that other objectives, allowing even transparent objects in the sample to be clearly identified.
Experience 3D Views Driven by the “Inversion Contrast” (IVC) Technique
With this newly developed IVC technique, the depth of field is narrower than that of the phase contrast making it possible to render clear three-dimensional images for objects of any shape or transparency. In addition, IVC observation provides clear views without halos or directional shadows, preserving the integrity of object details during observation.
User-Oriented Design for Efficient Cell Sampling and Handling
The design allows flexibility of using different containers, easy handling of any type of cell culture containers and smooth cell observations in sterile conditions.
High Contrast under Bright Conditions
The “Umbra Shield” is designed specifically for fluorescence observation using the CKX53. The shield efficiently blocks out room light, enhances the contrast of fluorescence, and enables clear fluorescence observation even under bright lab conditions. When using phase contrast, the Umbra Shield can be lifted up to pass light through to the sample.
Clear Views with a Wide Range of Fluorescence Dyes
With the CKX53 standard fluorescence set, even weak fluorescence signals can be viewed clearly with the aid of different integrated light sources such as a 100 W mercury lamp (U-LH100HG), a 130 W high-pressure mercury lamp (U-HGLGPS), and 3rd party LEDs. The same type of mirror unit as those offered with the IX3 and BX3 microscopes can be set at three slots of the mirror unit slider.
Image courtesy of Olympus
Dimensions200 (W) x 498 (D) x 454 (H) mm (Phase Contrast Entry Configuration)
WeightApprox. 6.9 kg
|Operating Environment (Indoor Use)||
Operating Environment (Indoor Use)Ambient Temperature: 5 - 40 ºC (41 - 104 ºF), Maximum Relative Humidity: 80 % for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70 % at 34 ºC (93 ºF), 60 % at 37 ºC (99 ºF), to 50 % relative humidity at 40 ºC (104 ºF)
Manual CondenserUltra Long Working Distance Condenser: NA 0.3/ W.D. 72 mm (Built-in)
Revolving NosepieceBuilt-In 4 position
Observation MethodFluorescence (Blue/Green Excitations); Fluorescence (Ultraviolet Excitations); Phase Contrast; Inversion Contrast; Brightfield
IlluminatorTransmitted Illuminator - LED Lamp; Fluorescence Illuminator - Hg Lamp 100W; Light Guide Illumination