The HyperScope multiphoton imaging system now has advanced imaging capabilities; the introduction of an extended wavelength lens set means you can image deeper and through thin scattering layers in in vivo samples. Learn more here.
Hamamatsu Orca Flash 4.0 V3
Building on their extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the ORCA Flash 4.0 V3.
Calibrated for Quantitative Accuracy
Every Orca Flash 4.0 V3 is carefully calibrated as a precision instrument must be. This attention to detail delivers outstanding linearity, especially at low light, and offers improved photo response non-uniformity (PRNU) and dark signal non-uniformity (DSNU) to minimize pixel differences and reduce fixed pattern noise. Each camera ships with a certificate providing the read noise and photoelectron conversion factor specific for that camera.
Flexibility for Customized Data Control
Like its predecessors, each Orca Flash 4.0 V3 is capable of both USB 3.0 or Camera Link output. In addition, the Orca Flash 4.0 V3 offers data reduction through user-controllable look up tables (LUT) for 12 or 8-bit output. These two choices, combined with region of interest selection enable you to fine tune acquisition speed and image data requirements.
Patented Tools for Advanced Imaging
The Orca Flash 4.0 V3 includes the Lightsheet Readout Mode which takes advantage of sCMOS rolling shutter readout to enhance the quality of lightsheet images.
When paired with Hamamatsu's W-VIEW GEMINI image splitting optics, a single Orca Flash 4.0 V3 camera becomes a powerful dual wavelength imaging device. In “W-VIEW Mode,” each half of the sensor can be exposed independently, facilitating balanced dual colour imaging with a single camera. This feature can be combined with the new and patented “Dual Lightsheet Mode” making simultaneous dual wavelength lightsheet microscopy a reality. Finally, the Orca Flash 4.0 V3 is the perfect complement to their new W-VIEW GEMINI-2C dual camera, super resolution-quality, image splitting optics.
Powerful Triggering for Synchronization
Joining a full complement of sophisticated triggering routines, Hamamatsu's new Master Pulse timing generator allows the Orca Flash 4.0 V3 to truly run the experiment. Performing functions that previously would have required an external pulse generator the Orca Flash 4.0 V3’s Master Pulse has flexible timing delays built in. Powerful synchronization of multiple cameras and devices just became a lot simpler.
Quantum efficency82 % (peak QE: @ 560 nm)
|Effective no. of pixels||
Effective no. of pixels2048 (H)×2048 (V)
Cell size6.5 μm×6.5 μm
Effective area13.312 mm×13.312 mm
|Full well capacity||
Full well capacity30 000 electrons (typ.)
Readout speed100 frames/s (Full resolution, standard scan, Camera Link)
|40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit)|
|53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit)|
|80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit)|
Readout noiseStandard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median)
|Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median)|
Cooling methodPeltier cooling
Cooling temperatureForced air (Ambient at +20 ℃): -10 ℃
|Water (+20 ℃): -10 ℃|
|Water (+15 ℃): -30 ℃|
Dark current0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.)
|0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.)|
|0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.)|
Dynamic range37 000:1 (typ.)
InterfaceCamera Link / USB 3.0
A/D converter16 bit / 12 bit / 8 bit
Readout ModesNormal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet
|Master Pulse Generator (Pulse Modes)||
Master Pulse Generator (Pulse Modes)Internal Sync, Start Trigger, Burst
|Master Pulse Generator (Pulse Interval in 1 μs increments)||
Master Pulse Generator (Pulse Interval in 1 μs increments)10 μs to 10 s
|Dark Signal Non-Uniformity (DSNU)||
Dark Signal Non-Uniformity (DSNU)0.3 electrons rms
|Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)||
Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)0.06 % rms
|Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)||
Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)0.3 % rms
|Linearity error, full light range (EMVA 1288 standard)||
Linearity error, full light range (EMVA 1288 standard)0.5 %
|Linearity error, low light range (< 500 electrons signal)||
Linearity error, low light range (< 500 electrons signal)0.2 % / Less than approx. 1 electron absolute error