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Photometrics Prime 95B Scientific CMOS Camera
Prime 95B is a Scientific CMOS camera with extreme sensitivity using high Quantum Efficiency (QE) Backside Illumination (BSI), a first for Scientific CMOS cameras.
High peak quantum efficiency
The 95B’s sensor converts up to 95% of incident photons into a measurable signal. Unlike microlens approaches to increasing QE, which lose effectiveness as objective magnification is increased, Prime 95B’s BSI sensor brings light into the pixel photodiode from behind, avoiding structures that reflect or absorb light. When combined with large 11µm pixels, Prime 95B can deliver over 300% more signal than other sCMOS cameras at 100X magnification.
Better than EMCCD
Prime 95B outperforms EMCCD cameras—with no excess noise that negates the benefit of using a high QE sensor, and additional limitations from EM gain calibration, stability, expense, and sensor lifetime. With a true 16-bit dynamic range, Prime 95B easily accomplishes what EMCCD can not—detect weak and bright signals within the same image with photon-noise limited performance.
The extreme sensitivity not only allows fainter signals to be detected, it provides the flexibility to increase frame rates, or turn down the excitation intensity to reduce cellular photo-damage. Yet Prime 95B maintains the same high frame rates, field-of-view and extremely low read noise that has made sCMOS so popular for live-cell imaging.
Primary Application and Techniques
- Super-Resolution Microscopy
- Confocal Microscopy
- Single Molecule Fluorescence
- Lightsheet Microscopy
Read Noise1.6e- (Median)
Pixel Area11µm x 11µm
Frame Rates41fps @ 16-bit / 82fps @ 12-bit
|Active Array Size||
Active Array Size1200 x 1200 pixels (1.44 Megapixel)
SensorGPixel GSense 144 BSI CMOS Gen IV, Grade 1 in imaging area
Sensor Area13.2mm x 13.2mm
Full-Well Capacity80,000e- (Combined Gain)
|4,500e- (High Gain)|
Bit Depth16-bit (Combined Gain)
|12-bit (High Gain)|