Photometrics Prime 95B Scientific CMOS Camera

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Photometrics Prime 95B Scientific CMOS Camera

Prime 95B is a Scientific CMOS camera with extreme sensitivity using high Quantum Efficiency (QE) Backside Illumination (BSI), a first for Scientific CMOS cameras.

Photometrics Prime 95B Scientific CMOS Camera

High peak quantum efficiency

The 95B’s sensor converts up to 95% of incident photons into a measurable signal. Unlike microlens approaches to increasing QE, which lose effectiveness as objective magnification is increased, Prime 95B’s BSI sensor brings light into the pixel photodiode from behind, avoiding structures that reflect or absorb light. When combined with large 11µm pixels, Prime 95B can deliver over 300% more signal than other sCMOS cameras at 100X magnification.


Better than EMCCD

Prime 95B outperforms EMCCD cameras—with no excess noise that negates the benefit of using a high QE sensor, and additional limitations from EM gain calibration, stability, expense, and sensor lifetime. With a true 16-bit dynamic range, Prime 95B easily accomplishes what EMCCD can not—detect weak and bright signals within the same image with photon-noise limited performance.

Extreme Sensitivity


The extreme sensitivity not only allows fainter signals to be detected, it provides the flexibility to increase frame rates, or turn down the excitation intensity to reduce cellular photo-damage. Yet Prime 95B maintains the same high frame rates, field-of-view and extremely low read noise that has made sCMOS so popular for live-cell imaging.

Schematics

Primary Application and Techniques

  • Super-Resolution Microscopy
  • Confocal Microscopy
  • Single Molecule Fluorescence
  • Lightsheet Microscopy

Schematics

Primary Application and Techniques

  • Super-Resolution Microscopy
  • Confocal Microscopy
  • Single Molecule Fluorescence
  • Lightsheet Microscopy

Read Noise
Read Noise
1.6e- (Median)
1.8e- (RMS)
Quantum Efficiency
Quantum Efficiency
95%
Pixel Area
Pixel Area
11µm x 11µm
Dynamic Range
Dynamic Range
61,500:1
Frame Rates
Frame Rates
41fps @ 16-bit / 82fps @ 12-bit
Active Array Size
Active Array Size
1200 x 1200 pixels (1.44 Megapixel)
Sensor
Sensor
GPixel GSense 144 BSI CMOS Gen IV, Grade 1 in imaging area
Sensor Area
Sensor Area
13.2mm x 13.2mm
18.7mm diagonal
Full-Well Capacity
Full-Well Capacity
80,000e- (Combined Gain)
4,500e- (High Gain)
Bit Depth
Bit Depth
16-bit (Combined Gain)
12-bit (High Gain)
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