Molecular Devices Axopatch 200B Amplifier

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Molecular Devices Axopatch 200B Amplifier

The benchmark for single-channel recordings and other applications where an excellent signal-to-noise ratio is key

For single-channel recording, whole-cell voltage- and current-clamp recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore and voltammetry studies

Molecular Devices Axopatch 200B Amplifier
Widely accepted as the gold-standard

For ultra-low noise patch clamp recordings, the Axopatch™ 200B Capacitor Feedback Patch Clamp Amplifier is the premier microelectrode amplifier for ultra-low noise patch

Incorporates capacitor-feedback technology and active headstage cooling

For single-channel recording, while resistive-feedback circuitry is used for two different ranges of whole-cell recording.

Two different speeds to optimize membrane potential recording

It also allows correction for voltage errors due to pipette resistance. This makes the Axopatch 200B System the amplifier of choice for a scientist who performs basic res

Integrating headstage mode

For measuring sub-picoamp currents to enable high-fidelity single-channel recordings from cell membranes and artificial bilayers

Resistive headstage mode

For larger currents and noise levels in whole-cell recordings

Slim headstage design

For easier access to the preparation on a crowded microscope stage

Series resistance compensation:

Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential.

Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance.

Dual-speed current clamp

Dual-speed current clamp optimizes speed and stability through better matching of the clamp algorithm to experimental parameters.

Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters.

Dual command potentials

Dual command potentials add flexibility by processing input signals from two different sources.

Selectable holding command input with two internal range settings and an external input enable convenient control of the output waveform.

Pipette capacitance compensation

Pipette capacitance compensation neutralizes the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry.

Cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings.

Selectable output gain

Selectable output gain with 10 settings to scale the output signal to the desired level.

Leak subtraction for correction of leak currents without software-based algorithms.

4-pole Bessel low-pass filter

Sophisticated 4-pole Bessel low-pass filter acts as anti-aliasing filter and can be used to pre-condition the output signal.

Configurable panel meter displays user-selectable parameters in real time for quick monitoring of the experiment.

User guide written by scientific consultants

Zap facilitates patch rupturing by applying a voltage pulse for a user-defined duration.

Two included pipette holders of different barrel lengths accept glass pipettes in most standard dimensions.

User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference.

In combination with a Digidata® 1550 Data Acquisition System and pCLAMP™ Software, the Axopatch 200B Amplifier forms the foundation of a patch clamp setup like those used in many electrophysiology labs around the world. Telegraphing between the software and the amplifier ensure correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the many advanced features of the Axopatch 200B Amplifier.

Dimensions (in.)
Dimensions (in.)
3.5 (H) x 19 (W) x 12.5 (D)
Dimensions (cm)
Dimensions (cm)
8.9 (H) x 48.3 (W) x 31.7 (D)
Weight
Weight
11.5 (5.1 kg)
Headstage (in.)
Headstage (in.)
0.75 (H) x 0.70 (W) x 4.2 (D)
Headstage (cm)
Headstage (cm)
1.8 (H) x 1.9 (W) x 10.5 (D)
Mounting plate (in.)
Mounting plate (in.)
0.25 (H) x 2.0 (W) x 2.5 (D)
Mounting plate (cm)
Mounting plate (cm)
0.6 (H) x 5.0 (W) x 6.2 (D)
Communications
Communications
Analog and digital BNC
Rack use
Rack use
Standard 19" rack-mount (2U) with handles
Benchtop use
Benchtop use
Standard 19" rack-mount (2U) with handles
Benchtop use
Benchtop use
Bayonet feet
Power
Power
85-264 VAC (110-340 VDC)50-60 Hz, 30 W (max)
Fuse
Fuse
0.5 A slow (15 x 20 mm)
Line filter
Line filter
RFI filter included
Line cord
Line cord
Shielded line cord provided
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