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Job Title: Postdoctoral Research Assistant
Location: Coventry, UK
Closing Date: 28th September 2021
The University of Warwick are seeking a highly determined and motivated candidate to join a team working to investigate a novel hypercapnic arousal pathway in the mammalian brain. Arousal in response to raised CO2 is a key survival pathway. They have found that CO2 sensitive connexin 26 (Cx26) hemichannels are expressed by GABAergic neurons in the VTA. They propose that the opening of these hemichannels by raised CO2 levels will shunt the GABAergic neurons removing inhibition and leading to arousal. This BBSRC-funded project will use a number of approaches to investigate this pathway, including a dominant negative construct to remove CO2-sensitivity from the Cx26 hemichannels expressed in the VTA neurons.
The Research Fellow will initially use the dominant negative construct to confirm that the CO2 sensitivity of VTA neurons is from Cx26 hemichannel expression. The construct will then be used in in vivo experiments to determine effects on arousal in response to raised levels of CO2. By using a variety of tracing methods combined with whole cell path clamp recording, the components of this novel arousal circuit will be fully defined.
For more information on the lab, click here
The successful Research Fellow candidate will have a PhD in a relevant area of experimental sciences, a career-appropriate track record of publishing high quality papers, and a strong intellectual interest in understanding neurophysiological signalling mechanisms by tracing signalling pathways from fundamental mechanism through to the final physiological output. The Research Fellow post requires a diverse skill-set. The successful candidate should be keen to learn new techniques but should have expertise in whole cell patch clamp recording methods, imaging with fluorescence/confocal microscopy. Some molecular biology knowledge, possession of a personal licence and experience of in vivo procedures operating under a UK Home Office licence will be an advantage