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Institute: Institute of Metabolic Science, University of Cambridge
Location: Cambridge, UK
Closing date: 12/01/2020
Start date: 16/03/2020
The laboratory of Dr. Florian Merkle (www.merklelab.org) is seeking a postdoc experienced in electrophysiology and/or calcium imaging to characterise cellular responses to nutrients, hormones, and drugs. Our group is interested in neuron populations that regulate food intake and that are critically important for the pathogenesis and treatment of obesity. In particular, we will generate these hypothalamic neurons in vitro from human pluripotent stem cells (hPSCs) and/or study them in mice. The successful applicant will characterise cellular responses to exogenous factors and test how mutations introduced by CRISPR/Cas9 affect cellular physiology and responsiveness. These studies will be performed at multiple scales, from the detailed analysis of individual cells by patch clamp electrophysiology to high-throughput screening for drug responses.
We are based at the Wellcome Trust-MRC Institute of Metabolic Science (https://www.ims.cam.ac.uk/) at the University of Cambridge, which provides a highly collaborative and interdisciplinary environment. The position is funded by the Wellcome Trust and Royal Society, and will provide the successful candidate with exposure cutting-edge techniques such as CRISPR/Cas9 gene editing, single-cell transcriptomics, and drug screening.
To be considered for this position, you must:
- Possess a PhD in neuroscience or a related field
- Be able to independently manage your time and experiments to generate data for publication, as supported by a track record of impactful papers in peer-reviewed journals
- Be proficient in the generation and analysis of patch clamp electrophysiology and/or calcium imaging data, as supported by publications
- Be able to troubleshoot experiments and develop novel research tools and platforms
- Have a track record of leadership and effective teamwork.
The ideal applicant would also have practical expertise with human pluripotent stem cell culture and differentiation, optogenetics/chemogenetics, fibre photometry, confocal imaging, and/or high-throughput screening.
There is some flexibility regarding start date and contract duration.