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Scientifica Multiphoton Resonant System

Designed to acquire fast frame rates deep into tissues over the entire field of view.

Tailored to large back aperture objectives, the entire field of view can be quickly scanned to monitor the activity from a large number of cells.

Benefits

Resonant scanning mirror

The resonant system features an 8 kHz scan mirror that can take frames of 512 x 512 pixels at 30 frames per second.

Acoustic isolation

Our custom designed scan head enclosure integrates acoustic insulating foam to greatly reduce the audible noise created by the resonant motor without affecting perfomance

Pioneering slim design

A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easily convert between in vivo and in vitro

The SliceScope's unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.

Uniform spot size

The relay lens system within the scan head achieves highly accurate imaging of small structures and prevents vignetting (or loss of signal) at the edge of the image.

Multiphoton resonant in vivo configuration 360° view

Control options

The resonant system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.

Upgrade to the Scientifica ChromoFlex

Enhance the Multiphoton Imaging System by upgrading to the ChromoFlex.

The ChromoFlex replaces the above stage multiphoton detection unit (MDU) and enables simultaneous imaging of up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.

Example Setup

The images below show an in vivo Multiphoton Resonant Imaging System including a riser block, XY stage, launch optics and movable periscope.

Testimonials

“The Scientifica multiphoton system was very easy to use. I have touched multiphoton systems before with an interface that at first is slightly intimidating, but this is not the feeling I had with Scientfifca’s system. It’s very fast, the fast acquisition will help researchers in certain scenarios.”

Greg Strachan, University of Cambridge 

Participant - EMBO Course on Advanced Optical Microscopy for Cell Biology, 2018

Design & Specifications

Scanning mirror (y-galvo)
Scanning mirror (y-galvo)
3 x 5 mm 8315KL scanning mirror (MicroMax 671HP driver)
Scanning mirror (x-resonant)
Scanning mirror (x-resonant)
3 x 5 mm 8 kHz CRS series scanner from Cambridge Technologies
Scan speeds
Scan speeds
15 fps @ 1024 x 1024 px, 30 fps @ 512 x 512 px, 60 fps @ 512 x 256 px
Beam input height
Beam input height
280.6 mm
Input beam diameter
Input beam diameter
<3 mm
Beam diameter at back aperture
Beam diameter at back aperture
<20.1 mm
Beam expansion
Beam expansion
6.7x
Relay lens expansion
Relay lens expansion
1x
Lens coating
Lens coating
700-1200 nm (Rav <0.5%)
Maximum scan angles (galvo)
Maximum scan angles (galvo)
+/- 10 optical degrees
Maximum scan angles (scan head)
Maximum scan angles (scan head)
+/- 7.6 optical degrees
Galvo voltage/optical degree
Galvo voltage/optical degree
0.25 V
Turning mirror size
Turning mirror size
45 x 64 x 6 mm
Turning mirror coating
Turning mirror coating
Protected Silver (98% reflectivity)
Typical field of view
Typical field of view
~600 µm2 (16x), ~500 µm2 (20x)
Scan control
Scan control
SciScan, ScanImage 5

Downloads

Download the Multiphoton Resonant System brochure for more information.

In Vivo

Multiphoton Resonant System SchematicsMultiphoton Resonant System SchematicsMultiphoton Resonant System Schematics

In Vitro

Multiphoton Resonant System In Vitro SchematicsMultiphoton Resonant System In Vitro SchematicsMultiphoton Resonant System In Vitro Schematics

Steck, K., Walker, S., Itskov, P., Baltazar, C., Moreira, J. and Ribeiro, C. (2018). Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila. eLife, 7 https://doi.org/10.7554/eLife.31625.001 

Testimonials

“The Scientifica multiphoton system was very easy to use. I have touched multiphoton systems before with an interface that at first is slightly intimidating, but this is not the feeling I had with Scientifica’s system. It’s very fast, and fast acquisition will help researchers in certain scenarios.”


- Greg Strachan, University of Cambridge 

User at the EMBO Course on Advanced Optical Microscopy for Cell Biology, 2018


“I like the Scientifica microscope as it is very flexible, so you can easily change the configuration to adapt the microscope for your experiment. For example, if you want to change it from in vitro to in vivo you just need to remove the condenser”


- Dr. Feng Wang, Yves De Koninck Lab, CERVO Research Center

Accessories

Movable Periscope Bracket (MP-4010-30)

This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

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