Scientifica Multiphoton Resonant System
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    The HoloStim-3D seamlessly integrates with the HyperScope, an award-winning multiphoton imaging system, to create an industry-leading spatial light modulator (SLM) system for all-optical interrogation of neural networks with previously unachievable performance.

    Scientifica Multiphoton Resonant System

    Scientifica's Multiphoton Resonant Imaging System is designed to acquire fast frame rates deep into tissues over the entire field of view.

    Tailored to large back aperture objectives, the entire field of view can be quickly scanned to monitor the activity from a large number of cells.

    (Model MP-2050 & MP-2070)

    Benefits

    Resonant scanning mirror

    The resonant imaging system features an 8 kHz scan mirror that can take frames of 512 x 512 pixels at 30 frames per second.

    Acoustic isolation

    Our custom designed scan head enclosure integrates acoustic insulating foam to greatly reduce the audible noise created by the resonant motor without affecting perfomance

    Pioneering slim design

    A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

    Easily convert between in vivo and in vitro

    The SliceScope's unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.

    Uniform spot size

    The relay lens system within the scan head achieves highly accurate imaging of small structures and prevents vignetting (or loss of signal) at the edge of the image.

    Multiphoton Imaging Systems Buyer's Guide

    MP-2050

    (in vivo or in vitro)

    • Three-photon imaging
    • Two-photon imaging
    • Second harmonic generation
    • Fluorescence lifetime imaging

    MP-2070

    (in vivo)

    • Three-photon imaging
    • Two-photon imaging
    • Second harmonic generation
    • Fluorescence lifetime imaging

    Multiphoton resonant in vivo configuration 360° view

    Control options

    The resonant imaging system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.

    Upgrade to the Scientifica ChromoFlex

    Enhance the Multiphoton Resonant Imaging System by upgrading to the ChromoFlex.

    The ChromoFlex replaces the above stage multiphoton detection unit (MDU) and enables simultaneous imaging of up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.

    Example Setup

    The images below show an in vivo Multiphoton Resonant Imaging System including a riser block, XY stage, launch optics and movable periscope.

    Design & Specifications

    Scanning mirror (y-galvo)
    Scanning mirror (y-galvo)
    3 x 5 mm 8315KL scanning mirror (MicroMax 671HP driver)
    Scanning mirror (x-resonant)
    Scanning mirror (x-resonant)
    3 x 5 mm 8 kHz CRS series scanner from Cambridge Technologies
    Scan speeds
    Scan speeds
    15 fps @ 1024 x 1024 px, 30 fps @ 512 x 512 px, 60 fps @ 512 x 256 px
    Beam input height
    Beam input height
    280.6 mm
    Input beam diameter
    Input beam diameter
    <3 mm
    Beam diameter at back aperture
    Beam diameter at back aperture
    <20.1 mm
    Beam expansion
    Beam expansion
    6.7x
    Relay lens expansion
    Relay lens expansion
    1x
    Lens coating
    Lens coating
    700-1200 nm (Rav <0.5%)
    Maximum scan angles (galvo)
    Maximum scan angles (galvo)
    +/- 10 optical degrees
    Maximum scan angles (scan head)
    Maximum scan angles (scan head)
    +/- 7.6 optical degrees
    Galvo voltage/optical degree
    Galvo voltage/optical degree
    0.25 V
    Turning mirror size
    Turning mirror size
    45 x 64 x 6 mm
    Turning mirror coating
    Turning mirror coating
    Protected Silver (98% reflectivity)
    Typical field of view
    Typical field of view
    ~600 µm2 (16x), ~500 µm2 (20x)
    Scan control
    Scan control
    SciScan, ScanImage 5

    Downloads

    Download the Multiphoton Resonant System brochure for more information.

    In Vivo

    Multiphoton Resonant System SchematicsMultiphoton Resonant System SchematicsMultiphoton Resonant System Schematics

    In Vitro

    Multiphoton Resonant System In Vitro SchematicsMultiphoton Resonant System In Vitro SchematicsMultiphoton Resonant System In Vitro Schematics

    Testimonials

    “The Scientifica multiphoton system was very easy to use. I have touched multiphoton systems before with an interface that at first is slightly intimidating, but this is not the feeling I had with Scientifica’s system. It’s very fast, and fast acquisition will help researchers in certain scenarios.”


    - Greg Strachan, University of Cambridge 

    User at the EMBO Course on Advanced Optical Microscopy for Cell Biology, 2018


    “I like the Scientifica microscope as it is very flexible, so you can easily change the configuration to adapt the microscope for your experiment. For example, if you want to change it from in vitro to in vivo you just need to remove the condenser”


    - Dr. Feng Wang, Yves De Koninck Lab, CERVO Research Center

    Lin, Y., Mazo, M., Skaalure, S., Thomas, M., Schultz, S., Stevens, M. (2019). Activatable cell–biomaterial interfacing with photo-caged peptides. Chemical Science, 10, 1158-1167 https://pubs.rsc.org/en/conten...

    Steck, K., Walker, S., Itskov, P., Baltazar, C., Moreira, J. and Ribeiro, C. (2018). Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila. eLife, 7 https://doi.org/10.7554/eLife.31625.001 

    Verdugo, C.D., Myren-Svelstad, S., Deneubourg, C., Pelgrims, R., Muto, A., Kawakami, K., Jurisch-Yaksi, N., Yaksi, E. (2019). Glia-neuron interactions underlie state transitions to generalized seizures. bioRxiv https://doi.org/10.1101/509521

    Accessories

    Movable Periscope Bracket (MP-4010-30)

    This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

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