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Scientifica Multiphoton Resonant System
Designed to acquire fast frame rates deep into tissues over the entire field of view.
Tailored to large back aperture objectives, the entire field of view can be quickly scanned to monitor the activity from a large number of cells.
Multiphoton resonant in vivo configuration 360° view
The resonant system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.
Upgrade to the Scientifica ChromoFlex
Enhance the Multiphoton Imaging System by upgrading to the ChromoFlex.
The ChromoFlex replaces the above stage multiphoton detection unit (MDU) and enables simultaneous imaging of up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.
The images below show an in vivo Multiphoton Resonant Imaging System including a riser block, XY stage, launch optics and movable periscope.
“The Scientifica multiphoton system was very easy to use. I have touched multiphoton systems before with an interface that at first is slightly intimidating, but this is not the feeling I had with Scientfifca’s system. It’s very fast, the fast acquisition will help researchers in certain scenarios.”
Greg Strachan, University of Cambridge
Participant - EMBO Course on Advanced Optical Microscopy for Cell Biology, 2018
Design & Specifications
|Scanning mirror (y-galvo)||
Scanning mirror (y-galvo)3 x 5 mm 8315KL scanning mirror (MicroMax 671HP driver)
|Scanning mirror (x-resonant)||
Scanning mirror (x-resonant)3 x 5 mm 8 kHz CRS series scanner from Cambridge Technologies
Scan speeds15 fps @ 1024 x 1024 px, 30 fps @ 512 x 512 px, 60 fps @ 512 x 256 px
|Beam input height||
Beam input height280.6 mm
|Input beam diameter||
Input beam diameter<3 mm
|Beam diameter at back aperture||
Beam diameter at back aperture<20.1 mm
|Relay lens expansion||
Relay lens expansion1x
Lens coating700-1200 nm (Rav <0.5%)
|Maximum scan angles (galvo)||
Maximum scan angles (galvo)+/- 10 optical degrees
|Maximum scan angles (scan head)||
Maximum scan angles (scan head)+/- 7.6 optical degrees
|Galvo voltage/optical degree||
Galvo voltage/optical degree0.25 V
|Turning mirror size||
Turning mirror size45 x 64 x 6 mm
|Turning mirror coating||
Turning mirror coatingProtected Silver (98% reflectivity)
|Typical field of view||
Typical field of view~600 µm2 (16x), ~500 µm2 (20x)
Scan controlSciScan, ScanImage 5
Research Papers Expand
Steck, K., Walker, S., Itskov, P., Baltazar, C., Moreira, J. and Ribeiro, C. (2018). Internal amino acid state modulates yeast taste neurons to support protein homeostasis in Drosophila. eLife, 7 https://doi.org/10.7554/eLife.31625.001
“The Scientifica multiphoton system was very easy to use. I have touched multiphoton systems before with an interface that at first is slightly intimidating, but this is not the feeling I had with Scientifica’s system. It’s very fast, and fast acquisition will help researchers in certain scenarios.”
- Greg Strachan, University of Cambridge
User at the EMBO Course on Advanced Optical Microscopy for Cell Biology, 2018
“I like the Scientifica microscope as it is very flexible, so you can easily change the configuration to adapt the microscope for your experiment. For example, if you want to change it from in vitro to in vivo you just need to remove the condenser”
- Dr. Feng Wang, Yves De Koninck Lab, CERVO Research Center
Movable Periscope Bracket (MP-4010-30)
This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.