Scientifica HyperScope | Multiphoton Microscopy and Photostimulation

The HyperScope multiphoton imaging system now has advanced imaging capabilities; the introduction of an extended wavelength lens set means you can image deeper and through thin scattering layers in in vivo samples. Learn more here.

Scientifica HyperScope
Overview Technical Data

Scientifica HyperScope

Performance measurements

These measurements were taken with two HyperScope systems, employing a Nikon 16X 0.8 NA 3 mm WD objective at 850 nm. The measured field of view at a scan angle of ±15° was 1 mm. The Point Spread Function measurements (below) were carried out by imaging 0.2µm polystyrene fluorescent beads mounted on the surface of a coverslip. From this figure you can see how our unique relay lens system enables having comparable resolution across the field of view.

The relay lenses also provide a constant intensity across the field of view, which can be observed on both the 3D surface plot and the 2D plot profile. Field flatness was mapped using a homogeneous fluorescent sample. Please note that the field of view of 1 mm corresponds only to the top portion of the plot.

Standard System

We can customise this system to suit your unique experimental needs. Please contact us to discuss your requirements.

1 x Detector Unit

MDU, MDU XL, Chromoflex or FLIM detector

1 x HyperScope scan head

The scan mirrors on the imaging path can be configured as a galvo-galvo, resonant-galvo or galvo-galvo-resonant scan head.

1 x Filter Cube Module

A compact, flexible and efficient epifluorescence illuminator, optimised for use with LED light engines. Specifically designed for Scientifica microscopes, the FCM ensures optimal illumination of samples in all possible configurations.

Research Papers

  • Rhythmic light flicker rescues hippocampal low gamma and protects ischemic neurons by enhancing presynaptic plasticity

    Zheng, L., Yu, M., Lin, R. et al. Rhythmic light flicker rescues hippocampal low gamma and protects ischemic neurons by enhancing presynaptic plasticity. Nat Commun 11, 3012 (2020). https://doi.org/10.1038/s41467-020-16826-0

  • Regulation of synapse weakening through interactions of the microtubule associated protein tau with PACSIN1

    Regan, P., Mitchell, S, J., Kim, S-C. et al. Regulation of synapse weakening through interactions of the microtubule associated protein tau with PACSIN1. Journal of Neuroscience (2021). https://doi.org/10.1523/JNEUROSCI.3129-20.2021

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