Simultaneously image and photostimulate hundreds of individual cells in 3D, with precise targeting of cells across large areas of the brain. Introducing the HoloStim-3D.
Simultaneously image up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.
Large collection optics allow greater light gathering from the objective back aperture. This increases overall efficiency by up to 10% with the liquid light guide when compared to our standard multiphoton detection unit, for better detection of weak fluorescent signals.
The ChromoFlex can be controlled from Scientifica’s own SciScan software or by Vidrio Technologies ScanImage software.
ChromoFlex in action at the Francis Crick Institute
Odorant-induced calcium transients in the mouse olfactory bulb in vivo.
Periglomerular cells expressing the genetically encoded calcium indicator GCaMP6f following viral injection (left). Imaging acquired on a Scientifica VivoScope multiphoton system with a ChromoFlex detection unit. Note how clearly cell bodies and neurites can be recognised. Changes in fluorescence intensity of cells highlighted in the left image are displayed as single trace with the same colour coding on the right. The presentation of an odorant stimulus (Isoamyl acetate; timing indicated by the grey bar) induces a strong fluorescence increase in the cells surrounding the bottom glomerulus while cells in the upper glomerulus remain mostly silent.
Data kindly acquired and provided by Dr Tobias Ackels and Dr Andreas Schaefer of the Francis Crick Institute
Design & Specifications
|Number of channels||
Number of channelsTwo, three and four channel options
|Objective maximum exit aperture||
Objective maximum exit aperture20 mm diameter
Angular collection+/- 8 degrees maximum from 20 mm exit aperture
Detector typesHamamatsu H10770PA-40 GaAsP
Objective compatibilityM32X0.75, M27X0.75, M25 X0.75 and RMS threaded objectives
|Laser/Visible dichroic mirror||
Laser/Visible dichroic mirror665 nm long-pass dichroic, factory-fitted (60 x 40 x 1 mm)
|Laser blocking filter||
Laser blocking filter680nm short-pass filter, factory fitted
Spectral filtrationTo be specified with order and requires Scientifica filter cube which is compatible with standard fluorescence filter sets (25 mm ø filters; 24 X 36 x 1mm dichroic mirror)
PMT protectionIntegrated protection circuitry in H10770PA-40 and remote light senor.
Preamplifier bandwidth20MHz (resonant system) and 1MHz (galvo) with low noise linear power supply
|High voltage control||
High voltage controlManual and/or software control
Chromoflex with 4 PMTs
Chromoflex with 3 PMTs
Chromoflex with 2 PMTs