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Scientifica ChromoFlex

Simultaneously image up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.

Benefits

Modular and Upgradeable

Choose between a 2, 3 or 4 PMT system depending on your current budget and experimental requirements. Upgrade when necessary for more complex studies.

Remote placement

The two-metre liquid light guide enables flexible placement of the detection module remotely from the collection module.

XYZ adjustment for input/output calibration

Optimise the mounting position of the liquid light guide to the collection optics with X, Y and Z axis adjusters to generate the highest quality images.

Protective shutter

Move the shutter into place to protect the PMTs during transmitted imaging or high levels of ambient light.

Ambient light sensor

Automatically detects high levels of ambient light in the imaging room to switch off the PMTs to prevent damage to PMTs which could cause poorer image quality.

New objective slider provided

Use your current objectives or a wide range of new objectives with RMS, M25, M27 or M32 threads. Easily change your objectives as required.

Efficient

Large collection optics allow greater light gathering from the objective back aperture. This increases overall efficiency by up to 10% with the liquid light guide when compared to our standard multiphoton detection unit, for better detection of weak fluorescent signals.

Control options

The ChromoFlex can be controlled from Scientifica’s own SciScan software or by Vidrio Technologies ScanImage software.

ChromoFlex in action at the Francis Crick Institute

Odorant-induced calcium transients in the mouse olfactory bulb in vivo.

Periglomerular cells expressing the genetically encoded calcium indicator GCaMP6f following viral injection (left). Imaging acquired on a Scientifica VivoScope multiphoton system with a ChromoFlex detection unit. Note how clearly cell bodies and neurites can be recognised. Changes in fluorescence intensity of cells highlighted in the left image are displayed as single trace with the same colour coding on the right. The presentation of an odorant stimulus (Isoamyl acetate; timing indicated by the grey bar) induces a strong fluorescence increase in the cells surrounding the bottom glomerulus while cells in the upper glomerulus remain mostly silent.

Data kindly acquired and provided by Dr Tobias Ackels and Dr Andreas Schaefer of the Francis Crick Institute

Inspired by researchers, designed by Scientifica

Scientifica developed ChromoFlex to meet neuroscientists requirements for multichannel two-photon imaging. The technology is inspired by papers from Ducros et al. 2011, Zinter & Levine 2011, Leray et al. 2008 and Beaurepaire & Mertz 2002.

Downloads

Download the ChromoFlex brochure for more information.

Design & Specifications

Number of channels
Number of channels
Two, three and four channel options
Objective maximum exit aperture
Objective maximum exit aperture
20 mm diameter
Angular collection
Angular collection
+/- 8 degrees maximum from 20 mm exit aperture
Detector types
Detector types
Hamamatsu H10770PA-40 GaAsP
Objective compatibility
Objective compatibility
M32X0.75, M27X0.75, M25 X0.75 and RMS threaded objectives
Laser/Visible dichroic mirror
Laser/Visible dichroic mirror
665 nm long-pass dichroic, factory-fitted (60 x 40 x 1 mm)
Laser blocking filter
Laser blocking filter
680nm short-pass filter, factory fitted
Spectral filtration
Spectral filtration
To be specified with order and requires Scientifica filter cube which is compatible with standard fluorescence filter sets (25 mm ø filters; 24 X 36 x 1mm dichroic mirror)
PMT protection
PMT protection
Integrated protection circuitry in H10770PA-40 and remote light senor.
Preamplifier bandwidth
Preamplifier bandwidth
20MHz (resonant system) and 1MHz (galvo) with low noise linear power supply
Preamplifier gain
Preamplifier gain
100000
Output connection
Output connection
BNC
High voltage control
High voltage control
Manual and/or software control

Chromoflex with 4 PMTs


Chromoflex with 3 PMTs


Chromoflex with 2 PMTs


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