Scientifica ChromoFlex
Simultaneously image up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.
Efficient
Large collection optics allow greater light gathering from the objective back aperture. This increases overall efficiency by up to 10% with the liquid light guide when compared to our standard multiphoton detection unit, for better detection of weak fluorescent signals.
Control options
The ChromoFlex can be controlled by Vidrio Technologies ScanImage software.
ChromoFlex in action at the Francis Crick Institute
Odorant-induced calcium transients in the mouse olfactory bulb in vivo.
Periglomerular cells expressing the genetically encoded calcium indicator GCaMP6f following viral injection (left). Imaging acquired on a Scientifica VivoScope multiphoton system with a ChromoFlex detection unit. Note how clearly cell bodies and neurites can be recognised. Changes in fluorescence intensity of cells highlighted in the left image are displayed as single trace with the same colour coding on the right. The presentation of an odorant stimulus (Isoamyl acetate; timing indicated by the grey bar) induces a strong fluorescence increase in the cells surrounding the bottom glomerulus while cells in the upper glomerulus remain mostly silent.
Data kindly acquired and provided by Dr Tobias Ackels and Dr Andreas Schaefer of the Francis Crick Institute
Inspired by researchers, designed by Scientifica
Scientifica developed ChromoFlex to meet neuroscientists requirements for multichannel two-photon imaging. The technology is inspired by papers from Ducros et al. 2011, Zinter & Levine 2011, Leray et al. 2008 and Beaurepaire & Mertz 2002.
Downloads
Download the ChromoFlex brochure for more information.
Design & Specifications
| Number of channels | Two, three and four channel options |
|---|---|
| Objective maximum exit aperture | 20 mm diameter |
| Angular collection | +/- 8 degrees maximum from 20 mm exit aperture |
| Detector types | Hamamatsu H10770PA-40 GaAsP |
| Objective compatibility | M32X0.75, M27X0.75, M25 X0.75 and RMS threaded objectives |
| Laser/Visible dichroic mirror | 665 nm long-pass dichroic, factory-fitted (60 x 40 x 1 mm) |
| Laser blocking filter | 680nm short-pass filter, factory fitted |
| Spectral filtration | To be specified with order and requires Scientifica filter cube which is compatible with standard fluorescence filter sets (25 mm ø filters; 24 X 36 x 1mm dichroic mirror) |
| PMT protection | Integrated protection circuitry in H10770PA-40 and remote light senor. |
| Preamplifier bandwidth | 20MHz (resonant system) and 1MHz (galvo) with low noise linear power supply |
| Preamplifier gain | 100000 |
| Output connection | BNC |
| High voltage control | Manual and/or software control |
Schematics
Chromoflex with 4 PMTs
Chromoflex with 3 PMTs
Chromoflex with 2 PMTs
