npi electronic FiberOptoMeter

Simultaneously image and photostimulate hundreds of individual cells in 3D, with precise targeting of cells across large areas of the brain. Introducing the HoloStim-3D.

npi electronic FiberOptoMeter

The ”optical multimeter” for probing brain circuits. Optogenetic stimulation & fluorescence measurement via the same fibre.

npi electronic FiberOptoMeter
Dual-band excitation

450 - 490 nm (OGB1, GCamP, ChR2) 550 - 590 nm (ArchT) Fluorescence recording in 500 - 540 nm band

Ultrafast photodetector

Robustness against high-dose irradiation detecting fluorescence signal immediately after optogenetic stimulation

Flexible illumination options

Short pulses at high power (optogenetics) on top of constant low power illumination (fluorescence)

Applications

  • Localised measurement of physiological fluorescence signals (e.g. Ca2+ signals) from multiple sites in freely behaving animals including primates
  • Multi-colour optogenetic stimulation
  • Near simultaneous optical measurement of cellular activity and optogenetic stimulation
  • Chronic monitoring of physiological signals in disease model animals and drug testing
  • Enhanced localised photodetection for deep-tissue multiphoton imaging
  • Combined fluorescence recording with fMRI imaging
  • Can be used as a supplemental photodetector channel for multiphoton microscopy
  • Compatible with multi-mode SMA fibres including non-magnetic fibers for fMRI

Available central wavelengths (LED)
Available central wavelengths (LED)
450 nm, 470 nm
570 nm, 617 nm, 625 nm
Excitation band
Excitation band
450 490 nm (blue)
540 590 nm (orange)
Maximum light power at 200 µm fiber tip
Maximum light power at 200 µm fiber tip
110 mW/mm (blue)
65 mW/mm (orange)
Fibre interface
Fibre interface
SMA or FC/PC
Fluorescence detection band
Fluorescence detection band
500 - 540 nm (green)
Fluorescence signal output filter
Fluorescence signal output filter
200 Hz low-pass (add-on block)
Fluorescence signal output range
Fluorescence signal output range
0 ~ -5 V (negative polarity
Dimensions
Dimensions
Requires 2 module slots of EPMS chassis (npi electronic)
Power consumption
Power consumption
20 W

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