Scientifica Multiphoton Galvo System
Scientifica Multiphoton Galvo System
Overview Technical Data

Scientifica Multiphoton Galvo System

Schematics

In vivo, large back aperture - side

In vivo, large back aperture - top

In vivo, large back aperture - front

In Vitro, Large Back Aperture - side

In Vitro, Large Back Aperture - top

In Vitro, Large Back Aperture - front

Standard System

We can customise this system to suit your unique experimental needs. Please contact us to discuss your requirements.

1 x Galvanometer scan head
1 x Detector

MDU, MDU XL, Chromoflex or FLIM

1 x Sofware

Compatible with ScanImage, SciScan or custom software

1 x Launch optics

Transmitted or reflected launch optics as required

Scanning

  • Scanning mirrors: 3 x 5 mm 8315KL scanning mirrors (MicroMax 671HP Drivers)
  • Maximum scan speed: 2 kHz in bidirectional scan mode (4 fps @512x512 px), 1 kHz in unidirectional scanning mode (2 fps @512x512 px)
  • Maximum scan angles (galvo): MP-2000: +/- 10 optical degrees
  • Maximum scan angles (scan head): +/- 7.6 optical degrees
  • Scan Control: SciScan, ScanImage 3.8.1

Beam

  • Beam input height: MP-1000: 198 mm, MP-2000: 280.6 mm
  • Maximum beam input diameter: <3 mm
  • Beam expansion: MP-1000: 3.3X, MP-2000: 6.7X
  • Beam diameter at back aperture: MP-1000: <10 mm, MP-2000: <20.1 mm

Lenses

  • Relay lens expansion: 1x
  • Lens coating: 700-1200 nm (Rav <0.5%)

Mirrors

  • Turning mirror size: MP-1000: 26 x 36 x 3 mm, MP-2000: 45 x 64 x 6 mm
  • Turning mirror coating: Protected silver (98% reflectivity)

Other specs

  • Typical field of view: MP-1000: ~800 µm2 (16x), ~750 µm2 (20x)*, MP-2000: ~300 µm2 (40x)
  • Galvo voltage/optical degree: 0.25 V

Research Papers

  • Conformational signaling required for synaptic plasticity by the NMDA receptor complex.

    Aow, J., Dore, K., & Malinow, R. (2015). Conformational signaling required for synaptic plasticity by the NMDA receptor complex. Proceedings Of The National Academy Of Sciences, 112(47), 14711-14716. http://dx.doi.org/10.1073/pnas...

  • Dendritic NMDA spikes are necessary for timing-dependent associative LTP in CA3 pyramidal cells.

    Brandalise, F., Carta, S., Helmchen, F., Lisman, J., & Gerber, U. (2016). Dendritic NMDA spikes are necessary for timing-dependent associative LTP in CA3 pyramidal cells. Nature Communications, 7, 13480. http://dx.doi.org/10.1038/ncom...

  • Agonist binding to the NMDA receptor drives movement of its cytoplasmic domain without ion flow.

    Dore, K., Aow, J., & Malinow, R. (2015). Agonist binding to the NMDA receptor drives movement of its cytoplasmic domain without ion flow. Proceedings Of The National Academy Of Sciences, 112(47), 14705-14710. http://dx.doi.org/10.1073/pnas..

  • Neuronal Hyperactivity Recruits Microglial Processes via Neuronal NMDA Receptors and Microglial P2Y12 Receptors after Status Epilepticus.

    Eyo, U., Peng, J., Swiatkowski, P., Mukherjee, A., Bispo, A., & Wu, L. (2014). Neuronal Hyperactivity Recruits Microglial Processes via Neuronal NMDA Receptors and Microglial P2Y12 Receptors after Status Epilepticus. Journal Of Neuroscience, 34(32), 10528-10540. http://dx.doi.org/10.1523/jneu...

  • Re-evaluation of learned information in Drosophila.

    Felsenberg, J., Barnstedt, O., Cognigni, P., Lin, S., & Waddell, S. (2017). Re-evaluation of learned information in Drosophila. Nature, 544(7649), 240-244. http://dx.doi.org/10.1038/natu...

  • Spinal Microgliosis Due to Resident Microglial Proliferation Is Required for Pain Hypersensitivity after Peripheral Nerve Injury.

    Gu, N., Peng, J., Murugan, M., Wang, X., Eyo, U., & Sun, D. et al. (2016). Spinal Microgliosis Due to Resident Microglial Proliferation Is Required for Pain Hypersensitivity after Peripheral Nerve Injury. Cell Reports, 16(3), 605-614. http://dx.doi.org/10.1016/j.ce...

  • Three Small-Receptive-Field Ganglion Cells in the Mouse Retina Are Distinctly Tuned to Size, Speed, and Object Motion.

    Jacoby, J., & Schwartz, G. (2016). Three Small-Receptive-Field Ganglion Cells in the Mouse Retina Are Distinctly Tuned to Size, Speed, and Object Motion. Journal Of Neuroscience. http://dx.doi.org/10.1523/jneu...

  • An Amacrine Cell Circuit for Signaling Steady Illumination in the Retina.

    Jacoby, J., Zhu, Y., DeVries, S., & Schwartz, G. (2015). An Amacrine Cell Circuit for Signaling Steady Illumination in the Retina. Cell Reports, 13(12), 2663-2670. http://dx.doi.org/10.1016/j.ce...

  • Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging.

    Johnston, J., Ding, H., Seibel, S., Esposti, F., & Lagnado, L. (2014). Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging. The Journal Of Physiology, 592(22), 4839-4854. http://dx.doi.org/10.1113/jphy...

  • Neuronal network activity controls microglial process surveillance in awake mice via norepinephrine signaling.

    Liu, Y., Ying, Y., Li, Y., Eyo, U., Chen, T., Zheng, J., Umpierre, A., Zhu, J., Bosco, D., Dong, H., & Wu, LJ. (2019). Neuronal network activity controls microglial process surveillance in awake mice via norepinephrine signaling. Nature Neuroscience, 22, 1771 - 1781. https://doi.org/10.1038/s41593-019-0511-3

  • The inhibitory neurotransmitter GABA evokes long-lasting Ca2+ oscillations in cortical astrocytes.

    Mariotti, L., Losi, G., Sessolo, M., Marcon, I., & Carmignoto, G. (2015). The inhibitory neurotransmitter GABA evokes long-lasting Ca2+ oscillations in cortical astrocytes. Glia, 64(3), 363-373. http://dx.doi.org/10.1002/glia...

  • Cardinal Orientation Selectivity Is Represented by Two Distinct Ganglion Cell Types in Mouse Retina.

    Nath, A., & Schwartz, G. (2016). Cardinal Orientation Selectivity Is Represented by Two Distinct Ganglion Cell Types in Mouse Retina. Journal Of Neuroscience, 36(11), 3208-3221. http://dx.doi.org/10.1523/jneu...

  • Aversive Learning and Appetitive Motivation Toggle Feed-Forward Inhibition in the Drosophila Mushroom Body.

    Perisse, E., Owald, D., Barnstedt, O., Talbot, C., Huetteroth, W., & Waddell, S. (2016). Aversive Learning and Appetitive Motivation Toggle Feed-Forward Inhibition in the Drosophila Mushroom Body. Neuron, 90(5), 1086-1099. http://dx.doi.org/10.1016/j.ne...

  • Scaling up multiphoton neural scanning: The SSA algorithm.

    Schuck, R., Annecchino, L., & Schultz, S. (2014). Scaling up multiphoton neural scanning: The SSA algorithm. 2014 36Th Annual International Conference Of The IEEE Engineering In Medicine And Biology Society. http://dx.doi.org/10.1109/embc...

  • PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology.

    Sweeney, A., Fleming, K., McCauley, J., Rodriguez, M., Martin, E., & Sousa, A. et al. (2017). PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology. Scientific Reports, 7, 43606. http://dx.doi.org/10.1038/srep...

  • Activation of microglial P2Y12 receptor is required for outward potassium currents in response to neuronal injury.

    Swiatkowski, P., Murugan, M., Eyo, U., Wang, Y., Rangaraju, S., Oh, S., & Wu, L. (2016). Activation of microglial P2Y12 receptor is required for outward potassium currents in response to neuronal injury. Neuroscience, 318, 22-33. http://dx.doi.org/10.1016/j.ne...

  • Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology.

    Schultz, S., Chadderton, P., Agabi, O., Copeland, C., Morris, A., Annecchino, L. (2017). Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology. Neuron, 95(5), 1048-1055. http://dx.doi.org/10.1016/j.ne...

  • Coordinated activation of distinct Ca2+ sources and metabotropic glutamate receptors encodes Hebbian synaptic plasticity.

    Tigaret, C., Olivo, V., Sadowski, J., Ashby, M., & Mellor, J. (2016). Coordinated activation of distinct Ca2+ sources and metabotropic glutamate receptors encodes Hebbian synaptic plasticity. Nature Communications, 7, 10289. http://dx.doi.org/10.1038/ncom...

  • Microglial calcium signaling is attuned to neuronal activity in awake mice.

    Umpierre, A., Bystrom, L., Ying, Y., Liu, Y., Worrell, G. and Wu, LJ. (2020). Microglial calcium signaling is attuned to neuronal activity in awake mice. eLife, https://elifesciences.org/articles/56502

  • Near-Infrared-Induced Heating of Confined Water in Polymeric Particles for Efficient Payload Release.

    Viger, M., Sheng, W., Doré, K., Alhasan, A., Carling, C., & Lux, J. et al. (2014). Near-Infrared-Induced Heating of Confined Water in Polymeric Particles for Efficient Payload Release. ACS Nano, 8(5), 4815-4826. http://dx.doi.org/10.1021/nn50...

  • Effect of Ultrasound on the Vasculature and Extravasation of Nanoscale Particles Imaged in Real Time.

    Yemane, P., Åslund, A., Snipstad, S., Bjørkøy, A., Grendstad, K., & Berg, S, et al. (2019). Effect of Ultrasound on the Vasculature and Extravasation of Nanoscale Particles Imaged in Real Time. Ultrasound in Medicine & Biology, 45(11), 3028 - 3041. https://doi.org/10.1016/j.ultrasmedbio.2019.07.683

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