Scientifica Multiphoton Galvo System

Take a look at our buyer’s guide to see our full multiphoton range and all scan headframe and detector options available: Multiphoton Imaging Systems Buyer's Guide

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Scientifica Multiphoton Galvo System

Scientifica’s award-winning two-photon imaging system with a galvanometer scan head for the greatest flexibility to direct the laser to any position in the field of view. The multiphoton galvo system is available in a variety of configurations and suitable for two-photon and three-photon imaging, second harmonic generation and fluorescence lifetime imaging.

'Galvo' mirrors offer the benefit of variable scanning speeds, adjustable to suit each individual application. Highly flexible scan patterns such as arbitrary line scans or patterned point scanning (e.g. for uncaging experiments) can easily be achieved.

(Model MP-1000, MP-2000 & MP-100794)

Benefits

Deep imaging

Outstanding images of the finest structures deep within your sample. Thanks to multiphoton optical sectioning, easily produce stunning three-dimensional reconstructions.

Region of interest scanning and line scanning

Increase the yield and veracity of your results when studying small cellular structures, such as a spine or bouton, on a dendrite or axon.

Fast frame rates

The galvanometer scan mirrors facilitate frame rates of up to 2 or 4 frames per second with unidirectional or bi-directional scanning respectively at 512 x 512 pixels.

Pioneering slim design

A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easily convert between in vivo and in vitro

The SliceScope's unique removable sub-stage increases the range of experiments which can be carried out on one rig.

Uniform “spot size”

The relay lens system within the Scientifica scan head achieves highly accurate imaging of small structures and prevents a loss of signal at the edge of the image.

Upgradeable to FLIM

Compatible with the Scientifica FLIM Upgrade kit for simultaneous fluorescence intensity and fluorescence lifetime imaging, in up to two colour channels.

Extended wavelength range

The large back aperture objective scan heads are coated for wavelengths of 1300 nm and beyond, giving you more experimental flexibility and three-photon capability.

The Scientifica multiphoton galvo system is available for large or small back aperture objectives, the SliceScope or VivoScope frame and a range of detection modules including Chromoflex, FLIM, MDU and MDU XL. Take a look at our buyer’s guide to see our full multiphoton range and all scan headframe and detector options available.

Multiphoton Imaging Systems Buyer's Guide

Multiphoton Imaging System Brochure

MP-1000

(in vivo or in vitro)

  • Two-photon imaging
  • Second harmonic generation
  • Fluorescence lifetime imaging

MP-2000

(in vivo or in vitro)

  • Three-photon imaging
  • Two-photon imaging
  • Second harmonic generation
  • Fluorescence lifetime imaging

MP-100794

(in vivo)

  • Three-photon imaging
  • Two-photon imaging
  • Second harmonic generation
  • Fluorescence lifetime imaging

Fluorescent reporter expressed in cerebellar Purkinje cells following a zebrin-like pattern (Credit: Kevin Dorgans/Isope Lab - Institute of Cellular and Integrative Neurosciences)
Fluorescent reporter expressed in cerebellar Purkinje cells following a zebrin-like pattern (Credit: Kevin Dorgans/Isope Lab - Institute of Cellular and Integrative Neurosciences)
Photo credit: Dr Jason Jacoby, Schwartz Lab, Northwestern University
Photo credit: Dr Jason Jacoby, Schwartz Lab, Northwestern University

Photo credit: Dr Olivier Manzoni, INMED
Two-photon images taken in 300µm thick striatum slices in 90 days old mice. Red cells are expressing tdTomato under the control of the D1 receptor promoter. Photo credit: Dr Olivier Manzoni, INMED

Control options

The galvo system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.

Design & Specifications

Scanning mirrors
Scanning mirrors
3 x 5 mm 8315KL scanning mirrors (MicroMax 671HP Drivers)
Beam input height
Beam input height
MP-1000: 198 mm
MP-2000: 280.6 mm
Maximum beam input diameter
Maximum beam input diameter
<3 mm
Relay lens expansion
Relay lens expansion
1x
Beam expansion
Beam expansion
MP-1000: 3.3X
MP-2000: 6.7X
Beam diameter at back aperture
Beam diameter at back aperture
MP-1000: <10 mm
MP-2000: <20.1 mm
Lens coating
Lens coating
700-1200 nm (Rav <0.5%)
Maximum scan speed
Maximum scan speed
2 kHz in bidirectional scan mode (4 fps @512x512 px), 1 kHz in unidirectional scanning mode (2 fps @512x512 px)
Maximum scan angles (galvo)
Maximum scan angles (galvo)
MP-2000: +/- 10 optical degrees
Maximum scan angles (scan head)
Maximum scan angles (scan head)
+/- 7.6 optical degrees
Galvo voltage/optical degree
Galvo voltage/optical degree
0.25 V
Turning mirror size
Turning mirror size
MP-1000: 26 x 36 x 3 mm
MP-2000: 45 x 64 x 6 mm
Turning mirror coating
Turning mirror coating
Protected silver (98% reflectivity)
Typical field of view
Typical field of view
MP-1000: ~800 µm2 (16x)*, ~750 µm2 (20x)*
MP-2000: ~300 µm2 (40x)*
Scan Control
Scan Control
SciScan, ScanImage 3.8.1
*May not be achievable if scanning at high speeds for prolonged periods of time

Testimonials

We have been using two multiphoton imaging systems from Scientifica, and have been very pleased with their performance. The systems are robustly constructed and designed to be easily integrated with electrophysiology. We have also been very pleased with the after-sales support provided by Scientifica."
Professor Leon Lagnado, University of Sussex
"The simple design improved the detection efficiency by positioning the detectors very close to the microscope objective. Also, the system can scan the samples pretty rapidly, which helps the reduction of phototoxicity and photobleaching."
Dr Kim Dore, University of California

Schematics (In Vivo, Large Back Aperture)

Multiphoton Imaging System In Vivo SchematicsMultiphoton Imaging System In Vivo SchematicsMultiphoton Imaging System In Vivo Schematics

Schematics (In Vitro, Large Back Aperture)

Multiphoton Imaging System SchematicMultiphoton Imaging System Schematic

Multiphoton Imaging System


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Accessories

Movable Periscope Bracket (MP-4010-30)

This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

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