Scientifica Multiphoton Imaging System

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Scientifica Multiphoton Galvo System

Scientifica’s award-winning two-photon imaging system comes with a galvanometer scan head for the greatest flexibility to direct the laser to any position in the field of view.

'Galvo' mirrors offer the benefit of variable scanning speeds, adjustable to suit each individual application. Highly flexible scan patterns such as arbitrary line scans or patterned point scanning (e.g. for uncaging experiments) can easily be achieved.


Capture a specific region, improve speed and increase the amount of data collected

Rather than relying on averaging to improve signal-to-noise ratios, a reduced scan speed can achieve the same results with less bleaching and tissue damage.

Deep imaging

Outstanding images of the finest structures deep within your sample. Combine with optical sectioning to produce excellent three-dimensional models.

Region of interest scanning

Increase the yield and veracity of your results when studying small cellular structures, such as a spine or bouton, on a dendrite or axon.

Fast frame rates

The galvanometer scan mirrors facilitate frame rates of up to 2 or 4 frames per second with unidirectional or bi-directional scanning respectively at 512 x 512 pixels.

Pioneering slim design

A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easily convert between in vivo and in vitro

The SliceScope's unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.

Uniform “spot size”

The relay lens system within the Scientifica scan head achieves highly accurate imaging of small structures and prevents a loss of signal at the edge of the image.

Control options

The galvo system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.

Upgrade to the Scientifica ChromoFlex

Enhance the Multiphoton Imaging System by upgrading to the ChromoFlex.

The ChromoFlex replaces the above stage multiphoton detection unit (MDU) and enables simultaneous imaging of up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.


Download the Multiphoton Imaging System brochure for more information.

Design & Specifications

Scanning mirrors
Scanning mirrors
3 x 5 mm 8315KL scanning mirrors (MicroMax 671HP Drivers)
Beam input height
Beam input height
MP-1000: 198 mm
MP-2000: 280.6 mm
Maximum beam input diameter
Maximum beam input diameter
<3 mm
Relay lens expansion
Relay lens expansion
Beam expansion
Beam expansion
MP-1000: 3.3X
MP-2000: 6.7X
Beam diameter at back aperture
Beam diameter at back aperture
MP-1000: <10 mm
MP-2000: <20.1 mm
Lens coating
Lens coating
700-1200 nm (Rav <0.5%)
Maximum scan speed
Maximum scan speed
2 kHz in bidirectional scan mode (4 fps @512x512 px), 1 kHz in unidirectional scanning mode (2 fps @512x512 px)
Maximum scan angles (galvo)
Maximum scan angles (galvo)
MP-2000: +/- 10 optical degrees
Maximum scan angles (scan head)
Maximum scan angles (scan head)
+/- 7.6 optical degrees
Galvo voltage/optical degree
Galvo voltage/optical degree
0.25 V
Turning mirror size
Turning mirror size
MP-1000: 26 x 36 x 3 mm
MP-2000: 45 x 64 x 6 mm
Turning mirror coating
Turning mirror coating
Protected silver (98% reflectivity)
Typical field of view
Typical field of view
MP-1000: ~800 µm2 (16x)*, ~750 µm2 (20x)*
MP-2000: ~300 µm2 (40x)*
Scan Control
Scan Control
SciScan, ScanImage 3.8.1
*May not be achievable if scanning at high speeds for prolonged periods of time


We have been using two multiphoton imaging systems from Scientifica, and have been very pleased with their performance. The systems are robustly constructed and designed to be easily integrated with electrophysiology. We have also been very pleased with the after-sales support provided by Scientifica."
Professor Leon Lagnado, University of Sussex
"The simple design improved the detection efficiency by positioning the detectors very close to the microscope objective. Also, the system can scan the samples pretty rapidly, which helps the reduction of phototoxicity and photobleaching."
Dr Kim Dore, University of California

Cell Images

Kevin Dorgans - two-photon cell images

Fluorescent reporter expressed in cerebellar Purkinje cells following a zebrin-like pattern (Credit: Kevin Dorgans/Isope Lab - Institute of Cellular and Integrative Neurosciences)

Schematics (In Vivo, Large Back Aperture)

Multiphoton Imaging System In Vivo SchematicsMultiphoton Imaging System In Vivo SchematicsMultiphoton Imaging System In Vivo Schematics

Schematics (In Vitro, Large Back Aperture)

Multiphoton Imaging System SchematicMultiphoton Imaging System Schematic

Multiphoton Imaging System

Aow, J., Dore, K., & Malinow, R. (2015). Conformational signaling required for synaptic plasticity by the NMDA receptor complex. Proceedings Of The National Academy Of Sciences, 112(47), 14711-14716.

Brandalise, F., Carta, S., Helmchen, F., Lisman, J., & Gerber, U. (2016). Dendritic NMDA spikes are necessary for timing-dependent associative LTP in CA3 pyramidal cells. Nature Communications7, 13480.

Dore, K., Aow, J., & Malinow, R. (2015). Agonist binding to the NMDA receptor drives movement of its cytoplasmic domain without ion flow. Proceedings Of The National Academy Of Sciences, 112(47), 14705-14710.

Dore, K., Aow, J., & Malinow, R. (2015). Agonist binding to the NMDA receptor drives movement of its cytoplasmic domain without ion flow. Proceedings Of The National Academy Of Sciences, 112(47), 14705-14710.

Eyo, U., Peng, J., Swiatkowski, P., Mukherjee, A., Bispo, A., & Wu, L. (2014). Neuronal Hyperactivity Recruits Microglial Processes via Neuronal NMDA Receptors and Microglial P2Y12 Receptors after Status Epilepticus. Journal Of Neuroscience, 34(32), 10528-10540.

Felsenberg, J., Barnstedt, O., Cognigni, P., Lin, S., & Waddell, S. (2017). Re-evaluation of learned information in Drosophila. Nature544(7649), 240-244.

Gu, N., Peng, J., Murugan, M., Wang, X., Eyo, U., & Sun, D. et al. (2016). Spinal Microgliosis Due to Resident Microglial Proliferation Is Required for Pain Hypersensitivity after Peripheral Nerve Injury. Cell Reports16(3), 605-614.

Jacoby, J., & Schwartz, G. (2016). Three Small-Receptive-Field Ganglion Cells in the Mouse Retina Are Distinctly Tuned to Size, Speed, and Object Motion. Journal Of Neuroscience.

Jacoby, J., Zhu, Y., DeVries, S., & Schwartz, G. (2015). An Amacrine Cell Circuit for Signaling Steady Illumination in the Retina. Cell Reports, 13(12), 2663-2670.

Johnston, J., Ding, H., Seibel, S., Esposti, F., & Lagnado, L. (2014). Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging. The Journal Of Physiology, 592(22), 4839-4854.

Mariotti, L., Losi, G., Sessolo, M., Marcon, I., & Carmignoto, G. (2015). The inhibitory neurotransmitter GABA evokes long-lasting Ca2+ oscillations in cortical astrocytes. Glia, 64(3), 363-373.

Mariotti, L., Losi, G., Sessolo, M., Marcon, I., & Carmignoto, G. (2015). The inhibitory neurotransmitter GABA evokes long-lasting Ca2+ oscillations in cortical astrocytes. Glia, 64(3), 363-373.

Nath, A., & Schwartz, G. (2016). Cardinal Orientation Selectivity Is Represented by Two Distinct Ganglion Cell Types in Mouse Retina. Journal Of Neuroscience36(11), 3208-3221.

Perisse, E., Owald, D., Barnstedt, O., Talbot, C., Huetteroth, W., & Waddell, S. (2016). Aversive Learning and Appetitive Motivation Toggle Feed-Forward Inhibition in the Drosophila Mushroom Body. Neuron90(5), 1086-1099.

Schuck, R., Annecchino, L., & Schultz, S. (2014). Scaling up multiphoton neural scanning: The SSA algorithm. 2014 36Th Annual International Conference Of The IEEE Engineering In Medicine And Biology Society

Sweeney, A., Fleming, K., McCauley, J., Rodriguez, M., Martin, E., & Sousa, A. et al. (2017). PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology. Scientific Reports, 7, 43606.

Swiatkowski, P., Murugan, M., Eyo, U., Wang, Y., Rangaraju, S., Oh, S., & Wu, L. (2016). Activation of microglial P2Y12 receptor is required for outward potassium currents in response to neuronal injury. Neuroscience, 318, 22-33.

Schultz, S., Chadderton, P., Agabi, O., Copeland, C., Morris, A., Annecchino, L. (2017). Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology. Neuron, 95(5), 1048-1055.

Tigaret, C., Olivo, V., Sadowski, J., Ashby, M., & Mellor, J. (2016). Coordinated activation of distinct Ca2+ sources and metabotropic glutamate receptors encodes Hebbian synaptic plasticity. Nature Communications7, 10289.

Viger, M., Sheng, W., Doré, K., Alhasan, A., Carling, C., & Lux, J. et al. (2014). Near-Infrared-Induced Heating of Confined Water in Polymeric Particles for Efficient Payload Release. ACS Nano, 8(5), 4815-4826.


Movable Periscope Bracket (MP-4010-30)

This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

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