Scientifica Multiphoton Galvo System

Scientifica Multiphoton Galvo Imaging System

Scientifica Multiphoton Galvo System

For in vivo and in vitro imaging with the greatest flexibility, look no further than the award-winning Multiphoton Galvo System.

Available in a variety of configurations, this multiphoton imaging system enables you to perform deep two-photon and three-photon fluorescence imaging, and fluorescence lifetime imaging (FLIM). You can easily adjust scanning speeds and patterns to suit your individual application, making this flexible system ideal for a variety of imaging experiments.

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Product benefits

Flexible scanning arrangements

Adjustable to suit your needs. Increase the yield and accuracy of your results when studying small cellular structures, such as a spine or bouton on a dendrite or axon, with region of interest scanning and line scanning.

Deep imaging

Obtain outstanding images of the finest structures deep within your sample. Easily produce stunning three-dimensional reconstructions, thanks to multiphoton optical sectioning.

Fast frame rates

Capture fast biological processes; the galvanometer scan mirrors facilitate frame rates of up to 2 or 4 frames per second with unidirectional or bi-directional scanning respectively, at 512 x 512 pixels.

Pioneering slim design

The compact and flexible design of the Multiphoton Galvo System, with either the slimline SliceScope or VivoScope frame, means you can easily integrate imaging with other techniques, such as electrophysiology.

Easily convert between in vivo and in vitro

Perform all of your experiments on one rig; the SliceScope’s unique removable sub-stage means you can easily convert between different configurations.

Highly accurate imaging of small structures

Achieve highly accurate imaging of small structures with no loss of signal at the edge of the image with the uniform ‘spot size’, achieved by the relay lens system within the Scientifica galvo scan head.

Upgradeable to FLIM

Compatible with the Scientifica FLIM Upgrade Kit for simultaneous fluorescence intensity and fluorescence lifetime imaging, in up to two colour channels.

Perform three-photon imaging

The large back aperture objective scan heads are coated for wavelengths of 1300 nm and beyond, giving you more experimental flexibility and three-photon capability.

Configurable to suit your needs

The Scientifica Multiphoton Galvo System is available for large or small back aperture objectives, the SliceScope or VivoScope frame and a range of detection modules including FLIM, MDU and MDU XL.

Layer V pyramidal cells in the visual cortex, 2 month old Thyl-eGFP mouse (Jax 7788) using LUMPFLN40x courtesy of: Kate Smith - Feinberg School of Medicine, Northwestern University Javi Munoz-Cuevas - Gallo Research Center, UCSF

Applications

The versatile Multiphoton Galvo System enables you to perform the following experiments, both in vivo and in vitro:

Example data

Take a look at the outstanding data that can be captured using Scientifica's Multiphoton Galvo System.

In vivo microglial cells, tagged with GFP, in the cortex of 3 month old mouse. Courtesy of the Long-Jun Wu lab at Rutgers University.

Fluorescent reporter expressed in cerebellar Purkinje cells following a zebrin-like pattern (Credit: Kevin Dorgans/Isope Lab - Institute of Cellular and Integrative Neurosciences)

Photo credit: Dr Jason Jacoby, Schwartz Lab, Northwestern University

Two-photon images taken in 300µm thick striatum slices in 90 days old mice. Red cells are expressing tdTomato under the control of the D1 receptor promoter. Photo credit: Dr Olivier Manzoni, INMED

Scientifica services for this product

Free Lab Consultations

Your Scientifica specialist can help optimise your lab through a free lab consultation, providing advice on the equipment best suited to your planned experiments and lab space.

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Lab Move Service

Our lab move service includes the pack down and reinstallation of your electrophysiology or multiphoton imaging system.

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Installation & Technical Support

Our installation team can install your system and provide your lab with bespoke training, ensuring your equipment is up and running as soon as possible.

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Demonstrations

Scientifica offer product demonstrations, allowing you to ask questions, learn about the multiphoton system and its applications, and see it for yourself.

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Customisations

Our range of modular parts enable us to tailor our systems to suit your unique research. These can be customised at the point of purchase or later in the lab.

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Warranty & Exchange Programme

Scientifica offer a two-year warranty with all Scientifica manufactured goods and a one-year warranty for third party supplied goods. Giving you peace of mind when purchasing your Scientifica equipment.

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Testimonials

"We have been using two multiphoton imaging systems from Scientifica, and have been very pleased with their performance. The systems are robustly constructed and designed to be easily integrated with electrophysiology. We have also been very pleased with the after-sales support provided by Scientifica."

Professor Leon Lagnado
University of Sussex

"The simple design improved the detection efficiency by positioning the detectors very close to the microscope objective. Also, the system can scan the samples pretty rapidly, which helps the reduction of phototoxicity and photobleaching."

Dr Kim Dore
University of California