CoolLED pE-4000 Universal Illumination System

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CoolLED pE-4000

The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy.

CoolLED pE-4000

Rapid switching between LED wavelengths enables capture of high speed events

Individual LED wavelength selection

TTL & USB interfaces with imaging packages

Excitation filters can be fitted in optical path for controlled switching with no moving parts

Analogue for dynamic intensity control

Optical feedback for applications requiring higher stability

As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains. These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available. This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets. The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics. A dual mode control pod gives manual control in either White or Advanced modes. Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.

Light Delivery

The pE-4000 can be configured to deliver light to the microscope via a single liquid light guide or fiber

​Environment & Safety

LED products are more sustainable and energy efficient than conventional light sources. CoolLED's products have the following benefits:

  • No Mercury
  • Energy Efficient: 80% less power
  • Long lifetime
  • No bulb replacements
  • Reduced risk of eye damage
  • No special disposal regulations

​Warranty

System = 12 months, LEDs = 36 months

Accessories

  • pE-4000-EB25D

Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity

  • pE-4000-EFH-4

Set of four Excitation Filter holders (25mm dia.)

  • pE-4000-L-MODULE

Interchangeable Light Delivery Output module for LLG (3mm dia.)

  • pE-4000-F-MODULE

Interchangeable Light Delivery Output module for Fiber (SMA/FC fittings)

  • Fibers

CoolLED’s pE-4000 illumination system can be configured to accept a fiber via either an SMA or FC connector for connecting a wide range of fibers. The user can source the fiber independently or request supply from CoolLED by specifying:-

Diameter in um (AAAA)
Numerical Aperture in mm (0BB)
Length in metres (C)
Termination 1 i.e. SMA, FC or C60- cleaved 60mm bare (DDD)
Termination 2 i.e. SMA, FC or C60-cleaved 60mm bare (EEE)

Power requirements
Power requirements
110-240V a.c. 50/60Hz, 2.5A
Power consumption
Power consumption
Standby (i.e. no LEDs on)
Standby (i.e. no LEDs on)
Max 7W
Single wavelength operation
Single wavelength operation
Max 41W
Dual wavelength operation
Dual wavelength operation
Max 75W
Triple wavelength operation
Triple wavelength operation
Max 93W
Quad wavelength operation
Quad wavelength operation
Max 112W
Dimensions
Dimensions
pE-4000 Light Source
pE-4000 Light Source
150mm(w) x 220mm(d) x 260mm(h) - Weight 3.5kg
pE-4000 Control pod
pE-4000 Control pod
154mm(w) x 135mm(d) x 40mm(h) – Weight 0.95kg
pE-4000 Power Supply
pE-4000 Power Supply
164mm(w) x 64mm(d) x 35mm(h) – Weight 0.58kg
pE-Expansion Box
pE-Expansion Box
151mm(w) x 18mm(d) x 95mm(h) - Weight 0.34kg
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