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The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy.
Rapid switching between LED wavelengths enables capture of high speed events
Individual LED wavelength selection
TTL & USB interfaces with imaging packages
Excitation filters can be fitted in optical path for controlled switching with no moving parts
Analogue for dynamic intensity control
Optical feedback for applications requiring higher stability
As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains. These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.
CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available. This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.
With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets. The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.
The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics. A dual mode control pod gives manual control in either White or Advanced modes. Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.
The pE-4000 can be configured to deliver light to the microscope via a single liquid light guide or fiber
Environment & Safety
LED products are more sustainable and energy efficient than conventional light sources. CoolLED's products have the following benefits:
- No Mercury
- Energy Efficient: 80% less power
- Long lifetime
- No bulb replacements
- Reduced risk of eye damage
- No special disposal regulations
System = 12 months, LEDs = 36 months
Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity
Set of four Excitation Filter holders (25mm dia.)
Interchangeable Light Delivery Output module for LLG (3mm dia.)
Interchangeable Light Delivery Output module for Fiber (SMA/FC fittings)
CoolLED’s pE-4000 illumination system can be configured to accept a fiber via either an SMA or FC connector for connecting a wide range of fibers. The user can source the fiber independently or request supply from CoolLED by specifying:-
Diameter in um (AAAA)
Numerical Aperture in mm (0BB)
Length in metres (C)
Termination 1 i.e. SMA, FC or C60- cleaved 60mm bare (DDD)
Termination 2 i.e. SMA, FC or C60-cleaved 60mm bare (EEE)
Power requirements110-240V a.c. 50/60Hz, 2.5A
|Standby (i.e. no LEDs on)||
Standby (i.e. no LEDs on)Max 7W
|Single wavelength operation||
Single wavelength operationMax 41W
|Dual wavelength operation||
Dual wavelength operationMax 75W
|Triple wavelength operation||
Triple wavelength operationMax 93W
|Quad wavelength operation||
Quad wavelength operationMax 112W
|pE-4000 Light Source||
pE-4000 Light Source150mm(w) x 220mm(d) x 260mm(h) - Weight 3.5kg
|pE-4000 Control pod||
pE-4000 Control pod154mm(w) x 135mm(d) x 40mm(h) – Weight 0.95kg
|pE-4000 Power Supply||
pE-4000 Power Supply164mm(w) x 64mm(d) x 35mm(h) – Weight 0.58kg
pE-Expansion Box151mm(w) x 18mm(d) x 95mm(h) - Weight 0.34kg