Cairn OptoSplit II

Cairn OptoSplit II

The Cairn OptoSplit II emission image splitter is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths, polarisation states or other differentiated states.

Traditionally, dual channel imaging is performed using an electronic filter changer or an additional camera and beamsplitter, neither of which is ideal for all applications. The switching speed of an electronic filter changer limits the temporal resolution, whereas a second camera adds cost and complexity. The OptoSplit uses a unique rotating mirror cradle, which gives adjustable spatial separation, to ensure excellent image registration and features a fully adjustable rectangular aperture to enable cropped sensor imaging modes and reduced scatter.

The latest version uses our own lens design to support the Larger Sensors of scientific CMOS and some EMCCD cameras. The instruments have a correspondingly larger aperture and improved off-axis correction to give enhanced performance with all sensors.

Cairn OptoSplit II

Compact design

With integral C-mount input and output ports

Simple & precise controls

For image registration

Applications

• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, voltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Total Internal Reflection Fluorescence (TIRF)
• Spinning disk confocal
• Single Plane Illumination Microscopy (SPIM)
• Polarisation studies (anisotropy)
• 3D super resolution PALM/STORM (using cylindrical lenses)
• Simultaneous phase contrast / DIC and fluorescence


Magnifications available
Magnifications available
Angled and flat auxiliary drop ins for neutral density filtering, polarisers or chromatic correction• Supports 25mm filters• Supports 2mm thick Ultraflat dichroics along with our recommended size (26x38x2mm)• •
Sensor support
Sensor support
For sensors up to 18.8mm diagonal (13.3mm x 13.3mm sensors)
Filter support
Filter support
Supports 25mm filters
Dichroic support
Dichroic support
Supports 2mm thick Ultraflat dichroics along with Cairn's recommended size (26x38x2mm)
Optics
Optics
40mm diameter proprietary optics
Coatings
Coatings
425nm to 875nm coatings on all surfaces

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