X-Cite XLED1

The X-LED1 is the perfect illuminator for advanced fluorescence microscopy, offering superior power output for fluorescence excitation with narrow excitation bands and fast wavelength switching capacity.

The system allows ultimate flexibility with individual wavelength intensity control and fast pulsing with no warming up time required.

The LED illuminator system offers three standard wavelengths to excite the most commonly used fluorophores ; 385nm, 460nm and 525nm. There is also the option of 635nm as an additional wavelength, with further options to be launched shortly. The improved intensity allows researchers to use the lightsource for optogenetic projects as well.

All LED modules are guaranteed to illuminate without failure for 20,000 hours or 3 years. This is the only instrument which offers this guarantee.

The X-LED1 is the perfect illuminator for advanced fluorescence microscopy, offering superior power output for fluorescence excitation with narrow excitation bands and fast wavelength switching capacity.

The system allows ultimate flexibility with individual wavelength intensity control and fast pulsing with no warming up time required.

The LED illuminator system offers three standard wavelengths to excite the most commonly used fluorophores ; 385nm, 460nm and 525nm. There is also the option of 635nm as an additional wavelength, with further options to be launched shortly. The improved intensity allows researchers to use the lightsource for optogenetic projects as well.

All LED modules are guaranteed to illuminate without failure for 20,000 hours or 3 years. This is the only instrument which offers this guarantee.

The microscope specific condenser optics means a higher power at the sample plane (385nm, 460nm, 525nm, optional 635nm) compared to other LED's- allowing increased excitation power where it matters most.

Narrow LED bandwidths result in high signal to noise ratios and reduced crosstalk between fluorophores 

Instantaneous wavelength switching makes the X-LED1 useful for fast multi-color excitation and pulsing applications (down to 10µs)

Fast on/off switching - Eliminates vibration and latency of mechanical shutters, allowing precise, short exposure times to limit photobleaching and phototoxicity.

Unique Internal Pulse Generator – allows you to set an imaging protocol in the hardware which can be triggered by an external TTL command or via the hand controller. This means you don't have to wait for separate triggers to create a pulsing protocol.

Optimized X-Cite® Condensers- High efficiency coupling to a wide variety of microscopes currently available with X-Cite® products.

0.1% intensity control- Creates repeatable illumination and optimal imaging, allowing more accurate quantitative and ratiometric imaging.

Intuitive 7" touch screen control panel, allowing easy control and protocol setup

> X-Cite XLED1 Brochure

> X-Cite XLED1 Product Selection Guide

Optogenetics Case Study

Optogenetic techniques use the power of light in order to inhibit or activate genetically defined populations of cells without affecting the surrounding cells. This technique is widely used to activate or inactivate ion channels and pumps within cells in order to replicate changes that may occur if the channel function is disrupted due to a genetic or environmental change. Traditionally, optogenetics is conducted using a laser or lamp system as these are known for their high powers. In this recent study, we tested if an LED light source would be able to reliably activate the chloride pump halorhodopsin in embryonic zebrafish. 

Find out more about Optogenetics here:

> X-Cite Optogenetics Case Study Document