Scientifica Multiphoton Resonant System

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Scientifica Multiphoton Resonant System

Designed to acquire fast frame rates deep into tissues over the entire field of view.

Tailored to large back aperture objectives, the entire field of view can be quickly scanned to monitor the activity from a large number of cells.

Benefits

Resonant scanning mirror

The resonant system features an 8 kHz scan mirror that can take frames of 512 x 512 pixels at 30 frames per second.

Acoustic isolation

Our custom designed scan head enclosure integrates acoustic insulating foam to greatly reduce the audible noise created by the resonant motor without affecting perfomance

Pioneering slim design

A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easily convert between in vivo and in vitro

The SliceScope's unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.

Uniform spot size

The relay lens system within the scan head achieves highly accurate imaging of small structures and prevents vignetting (or loss of signal) at the edge of the image.

Multiphoton resonant in vivo configuration 360° view

Control options

The resonant system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.

Upgrade to the Scientifica ChromoFlex

Enhance the Multiphoton Imaging System by upgrading to the ChromoFlex.

The ChromoFlex replaces the above stage multiphoton detection unit (MDU) and enables simultaneous imaging of up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.

Design & Specifications

Scanning mirror (y-galvo)
Scanning mirror (y-galvo)
3 x 5 mm 8315KL scanning mirror (MicroMax 671HP driver)
Scanning mirror (x-resonant)
Scanning mirror (x-resonant)
3 x 5 mm 8 kHz CRS series scanner from Cambridge Technologies
Scan speeds
Scan speeds
15 fps @ 1024 x 1024 px, 30 fps @ 512 x 512 px, 60 fps @ 512 x 256 px
Beam input height
Beam input height
280.6 mm
Input beam diameter
Input beam diameter
<3 mm
Beam diameter at back aperture
Beam diameter at back aperture
<20.1 mm
Beam expansion
Beam expansion
6.7x
Relay lens expansion
Relay lens expansion
1x
Lens coating
Lens coating
700-1200 nm (Rav <0.5%)
Maximum scan angles (galvo)
Maximum scan angles (galvo)
+/- 10 optical degrees
Maximum scan angles (scan head)
Maximum scan angles (scan head)
+/- 7.6 optical degrees
Galvo voltage/optical degree
Galvo voltage/optical degree
0.25 V
Turning mirror size
Turning mirror size
45 x 64 x 6 mm
Turning mirror coating
Turning mirror coating
Protected Silver (98% reflectivity)
Typical field of view
Typical field of view
~600 µm2 (16x), ~500 µm2 (20x)
Scan control
Scan control
SciScan, ScanImage 5

Schematics (In Vivo)

Multiphoton Resonant System SchematicsMultiphoton Resonant System SchematicsMultiphoton Resonant System Schematics

Schematics (In Vitro)

Multiphoton Resonant System In Vitro SchematicsMultiphoton Resonant System In Vitro SchematicsMultiphoton Resonant System In Vitro Schematics

Worldwide Presence

Scientifica’s equipment is used in more than 50 countries worldwide. Some of our customers include; California Institute of Technology, University of Oxford, Stanford University, University of Cambridge, Massachusetts Institute of Technology, Harvard University, Princeton University, Imperial College London, University of Chicago, Johns Hopkins University, Yale University and over 700 more research institutions.

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2 Year Warranty

Scientifica offer a two-year warranty with all Scientifca manufactured goods and a one-year warranty for third party supplied goods. Giving you peace of mind when purchasing your Scientifica equipment.

Scientifica Exchange Programme

Scientifica Exchange gives you a convenient method for swapping your faulty equipment over for working instruments quickly, to keep you working even if something goes wrong.

In the event that a Scientifica product needs repairing or servicing then the faulty equipment may be exchanged for the same type of equipment from Scientifica’s service stock.

To ensure the service is fast and cost effective we will ship you the replacement part as soon as we can. Read more about the exchange programme here.

Downloads

Download the Multiphoton Resonant System brochure for more information.

Accessories

Movable Periscope Bracket (MP-4010-30)

This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

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