Scientifica Multiphoton Imaging System

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Scientifica Multiphoton Imaging System

Scientifica’s award-winning two-photon imaging system comes with a galvanometer scan head for the greatest flexibility to direct the laser to any position in the field of view.

'Galvo' mirrors offer the benefit of variable scanning speeds, adjustable to suit each individual application. Highly flexible scan patterns such as arbitrary line scans or patterned point scanning (e.g. for uncaging experiments) can easily be achieved.


Capture a specific region, improve speed and increase the amount of data collected

Rather than relying on averaging to improve signal-to-noise ratios, a reduced scan speed can achieve the same results with less bleaching and tissue damage.

Deep imaging

Outstanding images of the finest structures deep within your sample. Combine with optical sectioning to produce excellent three-dimensional models.

Region of interest scanning

Increase the yield and veracity of your results when studying small cellular structures, such as a spine or bouton, on a dendrite or axon.

Fast frame rates

The galvanometer scan mirrors facilitate frame rates of up to 2 or 4 frames per second with unidirectional or bi-directional scanning respectively at 512 x 512 pixels.

Pioneering slim design

A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easily convert between in vivo and in vitro

The SliceScope's unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.

Uniform “spot size”

The relay lens system within the Scientifica scan head achieves highly accurate imaging of small structures and prevents a loss of signal at the edge of the image.

Control options

The resonant system can be fully controlled by Scientifica's own SciScan software or by Vidrio Technologies ScanImage software.

Upgrade to the Scientifica ChromoFlex

Enhance the Multiphoton Imaging System by upgrading to the ChromoFlex.

The ChromoFlex replaces the above stage multiphoton detection unit (MDU) and enables simultaneous imaging of up to four different colour dyes with the increased sensitivity of GaAsP PMT detectors.

Design & Specifications

Scanning mirrors
Scanning mirrors
3 x 5 mm 8315KL scanning mirrors (MicroMax 671HP Drivers)
Beam input height
Beam input height
MP-1000: 198 mm
MP-2000: 280.6 mm
Maximum beam input diameter
Maximum beam input diameter
<3 mm
Relay lens expansion
Relay lens expansion
Beam expansion
Beam expansion
MP-1000: 3.3X
MP-2000: 6.7X
Beam diameter at back aperture
Beam diameter at back aperture
MP-1000: <10 mm
MP-2000: <20.1 mm
Lens coating
Lens coating
700-1200 nm (Rav <0.5%)
Maximum scan speed
Maximum scan speed
2 kHz in bidirectional scan mode (4 fps @512x512 px), 1 kHz in unidirectional scanning mode (2 fps @512x512 px)
Maximum scan angles (galvo)
Maximum scan angles (galvo)
MP-2000: +/- 10 optical degrees
Maximum scan angles (scan head)
Maximum scan angles (scan head)
+/- 7.6 optical degrees
Galvo voltage/optical degree
Galvo voltage/optical degree
0.25 V
Turning mirror size
Turning mirror size
MP-1000: 26 x 36 x 3 mm
MP-2000: 45 x 64 x 6 mm
Turning mirror coating
Turning mirror coating
Protected silver (98% reflectivity)
Typical field of view
Typical field of view
MP-1000: ~800 µm2 (16x)*, ~750 µm2 (20x)*
MP-2000: ~300 µm2 (40x)*
Scan Control
Scan Control
SciScan, ScanImage 3.8.1
*May not be achievable if scanning at high speeds for prolonged periods of time


We have been using two multiphoton imaging systems from Scientifica, and have been very pleased with their performance. The systems are robustly constructed and designed to be easily integrated with electrophysiology. We have also been very pleased with the after-sales support provided by Scientifica."
Professor Leon Lagnado, University of Sussex
"The simple design improved the detection efficiency by positioning the detectors very close to the microscope objective. Also, the system can scan the samples pretty rapidly, which helps the reduction of phototoxicity and photobleaching."
Dr Kim Dore, University of California

Schematics (In Vivo, Large Back Aperture)

Multiphoton Imaging System In Vivo SchematicsMultiphoton Imaging System In Vivo SchematicsMultiphoton Imaging System In Vivo Schematics

Schematics (In Vitro, Large Back Aperture)

Multiphoton Imaging System SchematicMultiphoton Imaging System Schematic

Multiphoton Imaging System

Worldwide Presence

Scientifica’s equipment is used in more than 50 countries worldwide. Some of our customers include; California Institute of Technology, University of Oxford, Stanford University, University of Cambridge, Massachusetts Institute of Technology, Harvard University, Princeton University, Imperial College London, University of Chicago, Johns Hopkins University, Yale University and over 700 more research institutions.


Cell Images

Kevin Dorgans - two-photon cell images

Fluorescent reporter expressed in cerebellar Purkinje cells following a zebrin-like pattern (Credit: Kevin Dorgans/Isope Lab - Institute of Cellular and Integrative Neurosciences)

2 Year Warranty

Scientifica offer a two-year warranty with all Scientifca manufactured goods and a one-year warranty for third party supplied goods. Giving you peace of mind when purchasing your Scientifica equipment.

Scientifica Exchange Programme

Scientifica Exchange gives you a convenient method for swapping your faulty equipment over for working instruments quickly, to keep you working even if something goes wrong.

In the event that a Scientifica product needs repairing or servicing then the faulty equipment may be exchanged for the same type of equipment from Scientifica’s service stock.

To ensure the service is fast and cost effective we will ship you the replacement part as soon as we can. Read more about the exchange programme here.


Download the Multiphoton Imaging System brochure for more information.

Research Papers

Near-infrared-induced heating of confined water in polymeric particles for efficient payload release

ACS Nano

View paper

Neuronal hyperactivity recruits microglial processes via neuronal NMDA receptors and microglial P2Y12 receptors after status epilepticus

The Journal of Neuroscience

View paper

Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging

Journal of Physiology

View paper

Agonist binding to the NMDA receptor drives movement of its cytoplasmic domain without ion flow

Proceedings of the National Academy of Sciences

View paper


Movable Periscope Bracket (MP-4010-30)

This bracket attaches the periscope directly to the XY stage allowing the customer to move the microscope in X and Y whilst maintaining perfect laser alignment.

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